BACKGROUND Immune-mediated hemolytic anemia (IMHA) is the most common hematologic immune-mediated disease in dogs. Complement fixation on erythrocytes causes hemolysis. Complement inhibition decreases hemolysis in people with the hemolytic disease and also may prove effective in treating IMHA in dogs. HYPOTHESIS/OBJECTIVES Evaluate the in vitro efficacy of 2 complement inhibitors used in huma...

BACKGROUND The analysis of sperm DNA fragmentation has become a new marker to predict male infertility, and many techniques have been developed. The sperm Comet assay offers the possibility of differentiating single- and double-stranded DNA (ssDNA and dsDNA) breaks, which could have different effects on fertility. The objective of this study was to perform a descriptive characterization of diff...

The classic plaque assay is a method for counting infectious viral particles, however its complexity limits its use in a variety of virological experiments. To simplify the operation and to improve the repeatability, we employed an improved plaque assay procedure based on Avicel to make the whole experiment easier and optimize the results on a model of Vero cells infection with Enterovirus 71(E...

The single cell gel electrophoresis (SCGE) assay, also known as the Comet assay, is one of the most promising genotoxicity tests developed in recent years to measure and analyse DNA damage in single cells. The present study was undertaken to assess the in vivo genotoxicity of the synthetic pyrethroid cypermethrin in brain ganglia and anterior mid gut of Drosophila melanogaster. Freshly emerged ...

Infectious bovine keratoconjunctivitis (IBK) is a highly contagious ocular disease that affects cattle of all ages and that occurs worldwide. Piliated hemolytic Moraxella bovis is recognized as the etiologic agent of IBK. According to data from the Nebraska Veterinary Diagnostic Laboratory System, however, Moraxella (Branhamella) ovis has been isolated with increasing frequency from cattle affe...

The article presents the results of a clinical and bacteriological assessment pharmacological efficacy 1,3-diethylbenzimidazolium triiodide in chronic compensated tonsillopharyngitis. As result 10-day course treatment, an improvement status patients was achieved, as well significant positive effect on composition pharyngeal microbiocenosis. In particular, content Staphylococcus aureus, β-hemoly...

background and objective: hemolytic uremic syndrome (hus) is the most prevalent cause of children renal insufficiency which in many cases (90%) occurs following diarrhea. hemolytic microangiopathic anemia, thrombocytopenia, and renal insufficiency are main symptoms of hemolytic uremic syndrome. this study aims to consider the relationship between pathologic data of nephro-biopsy and laboratory ...

introduction methylene blue was first described for the treatment of methemoglobinemia but practical usage of the compound for surgical purpose is common. the aim of this report is to describe a case of hemolysis in neonatal period as a potential hazard of methylene blue toxicity without presence of g6pd deficiency. case presentation in october 2015, a 36-week ga female infant with 2.05 kg weig...