The purpose of this study was to evaluate the impact of mitochondrial DNA (mtDNA) on radiation sensitivity under hypoxic conditions. The cell lines used were rho+ and rho0, which carry wild-type mtDNA and no mtDNA, respectively. The rho0 cells do not utilize oxygen because they lack the capacity to carry out oxidative phosphorylation. To confirm the role played by mtDNA in different cell lines,...

Introduction: In the point of physical view, there are no significant differences between tumor and normal tissues during radiation therapy. Radio-sensitizers have a key role to address the issue. Exploiting high atomic number, gold nanoparticles (GNPs) have been introduced as novel radio-sensitizers and have shown promising result in the field. Owing to high mass attenuation c...


 Purpose: To investigate the effect of microRNA-612 (miR-612) on human colon cancer cells, and mechanism involved.
 Methods: Expressions miR-612 GPR132 were determined by quantitative real-time polymerase chain reaction (qRT-PCR)el , while cell viability was evaluated using counting kit-8 (CCK8) colony formation assays. Dual luciferase assay used to determine interaction between GPR1...

Abstract Radiotherapy resistance is a challenge for colorectal cancer (CRC) treatment. Circular RNAs (circRNAs) play vital roles in the occurrence and development of CRC. This study aimed to investigate role circ_0005615 regulating radiosensitivity The levels circ_0005615, microRNA-665 (miR-665), notch receptor 1 (NOTCH1) were detected by quantitative real-time PCR or western blot. CRC cells wa...

The obstacle in delivering therapeutics to glioblastoma (GBM) is tumor-induced angiogenesis which leads to the formation of abnormal vessels and a dysfunctional blood-tumor barrier. Here, we elucidated the effect of endothelial-monocyte activating polypeptide II (EMAP II) on the GBM-induced angiogenesis as well as its potential mechanisms. Our results proved that EMAP II inhibited the viability...

1. The ability of myo-inositol polyphosphates to inhibit iron-catalysed hydroxyl radical formation was studied in a hypoxanthine/xanthine oxidase system [Graf, Empson and Eaton (1987) J. Biol. Chem. 262, 11647-11650]. Fe3+ present in the assay reagents supported some radical formation, and a standard assay, with 5 microM Fe3+ added, was used to investigate the specificity of compounds which cou...

results the minimum inhibitory concentrations (mics) of ginger extract for evaluated strains were 40, 40, 20, 20, 20, 20, 10, and 5 mg/ml for pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae, bacillus cereus, acinetobacter baumannii, c. albicans, and c. krusei, respectively. ginger extract successfully inhibited biofilm formation by a. baumannii, b. cereus,...

BACKGROUND Statin can induce the gene expression of bone morphogenetic protein-2. Red yeast rice (RYR, Hongqu), i.e. rice fermented with Monascus purpureus, contains a natural form of statin. This study demonstrates the effects of RYR extract on bone formation. METHODS Bone defects were created in the parietal bones of two New Zealand white rabbits. In the test animal, two defects were grafte...

Commercially available diagnostic tools for the detection of lactate dehydrogenase elevating virus (LDV) infection have been restricted to measurement of serum lactate dehydrogenase (LDH) activity levels and detection of the viral genome by RT-PCR assays. Serologic diagnosis of LDV infection has not been widely adopted due to the belief that the formation of antigen-antibody complexes and B-cel...