ObjectivesUndetectable or low-level hepatitis B virus (HBV) DNA and drug resistance mutations in patients may increase the risk of HBV transmission cause active viral replication other clinical problems. Here, we established a highly sensitive practical method for detection using polymerase chain reaction (PCR) -based CRISPR-Cas13a system (referred to as PCR-CRISPR) evaluated its capability sam...

Hepatitis B virus (HBV) DNA is prone to mutations because of the proofreading deficiencies of HBV polymerase. We have identified hepatocellular carcinoma (HCC) survival-associated HBV mutations in the X protein region of HBV DNA. In the present study, we extend our research to assess HCC survival-associated HBV mutations in the HBV precore/core (PreC/C) region. The PreC/C region was amplified a...

Drug resistance gene mutations in Hepatitis B virus (HBV) are the main reason for failure of currently used therapeutic nucleoside analogues. Two methods-Pyrosequencing and Sanger sequencing, are most commonly used for HBV genotyping and identification of its mutations, but their advantages of the two methods are undefined. Herein, the two methods were used to identify the HBV genotypes and dru...

The compartmentalization of viral variants in distinct host tissues is a frequent event in many viral infections. Although hepatitis B virus (HBV) classically is considered hepatotropic, it has strong lymphotropic properties as well. However, unlike other viruses, molecular evolutionary studies to characterize HBV variants in compartments other than hepatocytes or sera have not been performed. ...

Hepatocellular carcinoma (HCC) is a public health problem in developing countries where chronic HBV endemic. The objective of our study was to determine the prevalence KRAS and BRAF mutations patients with HCC. Mutations codons 12 13 V600E mutation gene were searched by HRM on Light Cycler 480 confirmed direct sequencing. A total 34 HCC underwent molecular testing for codon gene. Melting curve ...

DNA viruses, retroviruses and hepadnaviruses, such as hepatitis B virus (HBV), are vulnerable to genetic editing of single stranded DNA by host cell APOBEC3 (A3) cytidine deaminases. At least three A3 genes are up regulated by interferon-alpha in human hepatocytes while ectopic expression of activation induced deaminase (AICDA), an A3 paralog, has been noted in a variety of chronic inflammatory...

The natural history and treatment outcome of hepatitis B viruses (HBV) infection is largely dependent on genotype, subgenotype, and the presence or absence of virulence associated mutations. We have studied the prevalence of genotype and subgenotype as well as virulence and drug resistance associated mutations and prevalence of recombinant among HBV from Bangladesh. A prospective cross-sectiona...

BACKGROUND Occult infection with hepatitis B virus (HBV) is a type of chronic HBV infection that is characterized by the absence of a detectable hepatitis B surface antigen in the blood and by very low levels of HBV DNA in the blood and liver. The mechanisms leading to occult HBV infection remain poorly understood but include possible genetic mutations and deletions. Recently, it has been shown...

Hepatitis B virus (HBV) genotype and precore and basal core promoter (BCP) mutants in the patients with persistently normal alanine aminotransferase (ALT) and low serum HBV-DNA levels are unclear. The aim of this study was to determine HBV genotypes, precore and BCP mutations, and their association with chronic hepatitis and liver fibrosis in HBV-infected patients with persistently normal ALT, ...