Thermal stabilities of chicken, grey lag goose (Anser anser), turkey as avian hemoglobins (Hbs); and human, bovine, sheep and horse as mammalian Hbs in hemolysate form were investigated and compared with oxygen affinities taken from literature. The thermal stability was obtained from thermal profiles using temperature scanning spectrophotometry. The buffer conditions were 50 mM Tris, pH 7.2, an...

The mutant hemoglobin S (HbS) previously was reported to undergo accelerated autooxidation during incubation in vitro. However, subsequent observations have raised the possibility that this might be explained by adventitious association of molecular iron with HbS, rather than reflecting an inherent property of HbS. Using purified HbA and HbS obtained from genotypic HbAS donors, we found that th...

We previously reported that circulating hemoglobin (Hb) CC erythrocytes contain oxygenated HbC crystals with little or no HbF and that HbF inhibits in vitro crystallization of HbC. We now report that HbS accelerates in vitro crystallization of HbC. Crystals were formed in 1.8 mol/L potassium phosphate buffer, pH 7.4, at 30 degrees C and were counted in several time intervals with a hematocytome...

OBJECTIVES The aim of our study was to assess immunological changes in hospital employees vaccinated five years ago with a recombinant hepatitis B vaccine. MATERIALS AND METHODS 148 hospital employees were examined at different time periods during 5 years following a three-dose schedule of intramuscular immunization with a recombinant DNA hepatitis B vaccine (Engerix-B). RESULTS At the end ...

Human Immunodeficiency Virus (HIV),Hepatitis B virus (HBV) and C virus (HCV) are known to be transmitted through blood and blood products. Many studies have been performed to determine relationship between infectious diseases and blood groups. This study aims to detect the relationship between the HIV, HBV and HCV infections with ABO and Rh D blood groups in Haryana. After blood donation, blood...

OBJECTIVE The Hepatitis B virus genome persists in the nucleus of virus infected hepatocytes where it serves as template for viral mRNA synthesis. Epigenetic modifications, including methylation of the CpG islands contribute to the regulation of viral gene expression. The present study investigates the effects of spontaneous age dependent loss of hepatitis B surface protein- (HBs) expression du...

background: hepatitis b virus is one of the most common causes of chronic liver diseases. vaccination is the best way of prophylaxis and is of utmost importance. this study was conducted to compare the prophylactic effect of intradermal and intramuscular injection of hepatitis b vaccine. methods: a blind randomized clinical trial was performed among subjects who presented to the infectious dise...