The tropane alkaloid anisodamine ( 2) is obtained by 6 beta-hydroxylation of hyoscyamine ( 1). The application of this alkaloid in medicine is gaining attention due to the wide range of therapeutic applications described in addition to its anticholinergic activity. In this work, the production of anisodamine ( 2) by IN VITRO cultures of BRUGMANSIA CANDIDA (Argentinean and Colombian samples) was...

Roots of the medicinal plant Valeriana officinalis are well-studied for their various biological activities. We applied genetically transformed V. officinalis root biomass to exert control of Physarum polycephalum, an amoeba-based emergent computing substrate. The plasmodial stage of the P. polycephalum life cycle constitutes a single, multinucleate cell visible by unaided eye. The plasmodium m...

It is currently possible to transfer a biosynthetic pathway from a plant to another organism. This system has been exploited to transfer the metabolic richness of certain plant species to other plants or even to more simple metabolic organisms such as yeast or bacteria for the production of high added value plant compounds. Another application is to bioconvert substrates into scarcer or biologi...

BACKGROUND Plants within the Orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. Despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS We developed transient and stable transformation systems for Phtheirospermum jap...

Global warming is one of the most serious problems leading to changes in distribution areas species and biodiversity. Rhodiola quadrifida a rare plant with adaptogenic properties grows highlands narrow temperature range 2–15 °C. The aim our work was study growth content main metabolites two vitro cultures at temperatures 5, 15 25 Hairy roots calli were cultivated on agar medium for 28 days. max...

Hairy root culture is a promising approach to improve production of plant secondary metabolites. The genes, which are located in T-DNA root-inducing plasmid, regulate auxin sensitivity hairy roots. Therefore, this study was aimed the growth and rosmarinic acid Lavandula angustifolia Lateral branches roots were transferred ½ MS B5 liquid media. To assess sensitivity, indole-3-acetic (IAA) indole...

Transformed hairy roots induced on Solanum dulcamara plants by Ri plasmid of Agrobacterium rhizogenes strain 8196 and A4T, exhibited a higher levels of auxin and cytokinin activity as compared with that of non transformed control roots. Endogenous pbytohormones; auxins and cytokinins were determined in an ethyl acetate fractionated extracts using bioassay methods of Hordium coleoptile test for ...

Background & Aim:Oxidative stresses caused by free radicals are known to promote many diseases such as inflammation and cancer; therefore, any plant product that can inhibit free radical production can play an important role in preventing diseases. We aimed to compare the fracture rate and the scavenging activity of free radicals, and the antioxidant composition of rol...