Case 1. An increase in mortality occurred in a herd of 7000 free ranging Rhim gazelles (Gazella subgutturosa marica) in the UAE. Post-mortem revealed ruminal bloat and generalized haemorrhagic gastro-enteritis. Laboratory diagnosis confirmed Clostridium perfringens (direct smears and toxins found) enterotoxaemia and coli septicaemia. As this disease process was considered multifactorial, dietar...

The viral haemorrhagic septicaemia virus (VHSV) comprises 4 major genotypes and a number of subtypes with, in most cases, distinct geographical distribution. A quick and simple detection method that can discriminate the different genotypes is desirable for a quick and more efficient prevention of the spread of genotypes to new geographical areas. A monoclonal antibody (MAb) against VHSV genotyp...

Forty Pasteurella multocida strains of serotype B:2,5 were collected from cattle affected with haemorrhagic septicaemia, and 72 strains mainly of serotype A:3, were isolated from calves with changes typical of bronchopneumonia. The outer membrane proteins (OMPs) were prepared by extraction, with 1% sarcosyl from field strains and two reference strains. OMPs were separated electrophoretically at...

A commercial plant extract derived from olive tree leaf (Olea europaea) (LExt) and its major compound, oleuropein (Ole), inhibited the in vitro infectivity of the viral haemorrhagic septicaemia virus (VHSV), a salmonid rhabdovirus. Incubation of virus with LExt or Ole before infection reduced the viral infectivity to 10 and 30%, respectively. Furthermore, LExt drastically decreased VHSV titers ...

Synthetic T7-driven cDNA minigenomes containing the bacterial chloramphenicol acetyltransferase gene as a reporter were derived from the genome of two salmonid novirhabdoviruses, infectious haematopoietic necrosis virus (IHNV) and viral haemorrhagic septicaemia virus (VHSV). We showed that an exogenous IHNV RNA minigenome transfected into fish cells could be rescued following IHNV infection as ...