Entero-invasive Escherichia coli (EIEC) and shigellae were tested for contact-haemolysin (CH) with red blood cells (RBCs) of guinea-pig, rabbit, rat, mouse, monkey, man, sheep and chicken; all bacteria showed the best lysis with guinea-pig RBCs. The best culture medium for CH activity of shigellae was tryptic soy broth, and for EIEC it was casamino acid-yeast extract broth with 1 mM CaCl2. CH p...

Salmonella enterica serovar Typhi (S. typhi) causes typhoid fever. We show that exposure of S. typhi to neuroendocrine stress hormones results in haemolysis, which is associated with the release of haemolysin E in membrane vesicles. This effect is attributed to increased expression of the small RNA micA and RNA chaperone Hfq, with concomitant downregulation of outer membrane protein A. Deletion...

This report investigated the origin of H(2)S in a newborn boy with sulfhaemoglobin induced cyanosis, who died because of multiple organ failure. Frozen material was collected and studied after death. The results suggest that enzymes had been released from deteriorating organs into the blood and abdominal fluid, and that the reaction of one of these enzymes with sulfur containing amino acids mig...

The presence of coagulase-positive staphylococci in the pulmonary tract of patients with cystic fibrosis (CF) has been well established (Huang, Van Loon, and Sheng, 1961; Iacocca, Sibinga, and Barbero, 1963). Investigators (Halbert, di Sant' Agnese, and Kotek, 1960; Gladstone et al., 1962; Mudd et al., 1962; Quie and Wannamaker, 1964) have demonstrated serum antibodies to the microbial organism...

Fifty Aeromonas caviae strains from intestinal infection in different Cuban provinces were identified by the Aerokey II method and virulence factors were investigated. The strains did not produce haemolysins but other exoenzymes such as proteases, lipases, and DNases; additionally, all isolates adhered to the HEp-2 cell line by the Carrello method and this did not correlate with other virulence...

In a healthy women, vaginal ecosystem acts to provide a barrier to both new colonization by pathogenic organisms and overgrowth of organisms that are otherwise commensals. Diabetes is a proven predisposing factor for the imbalance in vaginal microbiota resulting vulvovaginal candidiasis. The present study was undertaken to find out prevalence of vaginal candidiasis in diabetic women. For the st...

Strains of E. coli from the faeces of patients with active ulcerative colitis and with colitis in remission were examined for haemolysin and necrotoxin production. Cultures from 63 patients with active colitis grew haemolytic E. coli in 23 (37%) as compared with 24 (21%) from 115 patients whose disease was in remission (p < 0.05). The corresponding proportions for necrotoxin-producing strains w...

BACKGROUND Coeliac disease is an immune-mediated enteropathology triggered by the ingestion of cereal gluten proteins. This disorder is associated with imbalances in the composition of the gut microbiota that could be involved in its pathogenesis. The aim of the present study was to determine whether intestinal Enterobacteriaceae populations of active and non-active coeliac patients and healthy...