AIMS The objective of this study was to determine if the faecal microbiome has an association with Escherichia coli O157:H7 prevalence and enumeration. METHODS AND RESULTS Pyrosequencing analysis of faecal microbiome was performed from feedlot cattle fed one of three diets: (i) 94 heifers fed low concentrate (LC) diet, (ii) 142 steers fed moderate concentrate (MC) diet, and (iii) 132 steers f...

Strains of Escherichia coli serotype O157:H7 are Vero cytotoxin-producing enteric pathogens which have been associated with sporadic cases and outbreaks of hemorrhagic colitis and with the hemolytic uremic syndrome in humans. In addition to toxin production, adherence of many pathogenic bacteria to intestinal mucosal surfaces is a critical primary step in the pathogenesis of diarrheal diseases....

Consumption of produce contaminated with Escherichia coli O157:H7 has resulted in cases of foodborne illness. We determined the efficacy of a mixture of three E. coli O157:H7-specific bacteriophages (ECP-100) in reducing the number of viable E. coli O157:H7 on contaminated fresh-cut iceberg lettuce and cantaloupe. E. coli O157:H7 was spot inoculated on lettuce pieces (9 cm2) with a population o...

A fed-batch, anaerobic culture system was developed to assess the behavior of Escherichia coli O157:H7 in a rumen-like environment. Fermentation medium consisted of either 50% (vol/vol) raw or sterile rumen fluid and 50% phosphate buffer. Additional rumen fluid was added twice per day, and samples were removed three times per day to simulate the exiting of digesta and microbes from the rumen en...

background: escherichia coli o157:h7 is a gram-negative and facultative anaerobic food-borne bacterial pathogen. the major purpose of this study was to evaluate physicochemical characteristics of nanoliposome garlic essential oil (eo) and its antibacterial effect on e. coli o157:h7. methods: nanoliposome garlic eo was prepared by ethanol injection method and its physicochemical properties were ...

The objective of this research was to provide the characterization and method for producing anti-E. coli O157:H7 antibodies in egg-laying hens and to determine if the antibody can restrain the proliferation of E. coli O157:H7 in-vitro. Selected antigenic fractions (whole cell, outer membrane protein and lipopolysaccharide (LPS)) from E. coli O157:H7 were injected to hens in order to produce ant...

Escherichia coli O157:H7 is a food-borne bacterium that causes hemorrhagic diarrhea and hemolytic uremic syndrome in humans. While cattle are a known source of E. coli O157:H7 exposure resulting in human infection, environmental reservoirs may also be important sources of infection for both cattle and humans. Bacteriophage-encoded Shiga toxins (Stx) carried by E. coli O157:H7 may provide a sele...

Enhancing the thermal destruction of Escherichia coli O157:H7 in ground beef patties using trans-cinnamaldehyde Trans-cinnamaldehyde (TC) is an active antimicrobial substance extracted from the spice cinnamon which offers meat processors a labelfriendly, non-artificial sanitising agent. The effectiveness of TC in the inactivation of the foodborne pathogen Escherichia coli 0157:H7 in undercooked...

pigeons can be carriers of some human pathogens and contribute to transmission and spread of drug resistant infectious agents to human. considering many people’s interests to keep pigeons, present study was conducted to pcr detection of escherichia coli o157:h7 that is responsible for outbreaks of human intestinal diseases and fatal haemolytic-uraemic syndrome worldwide and evaluation of resist...

BACKGROUND Escherichia coli O157:H7 and Shigella flexneri are well-known food-borne pathogens causing severe food poisoning at low infectious doses. Bacteriophages have been approved for food applications by the US Food and Drug Administration (FDA) and have been suggested as natural food preservatives to control specific food-borne pathogens. To develop a novel natural food preservative agains...