1. The use of ;marker' enzymes for investigating the contamination by endoplasmic reticulum of mitochondrial and synaptosomal (nerve-ending) fractions isolated from guinea-pig brain was examined. NADPH-cytochrome c reductase appeared to be satisfactory. With the synaptosomal preparation there was a non-occluded enzymic activity believed to arise from contaminating microsomes and an occluded for...

The carnitine acetyltransferase and glutamate dehydrogenase activities of guinea-pig liver and other tissues were estimated. Both enzymes are wholly mitochondrial, and can only be fully observed after disruption of the mitochondrion. Triton X-100 (0.1%) or freeze-drying revealed more activity than other methods tried. In mitochondria prepared and suspended in 0.25m-sucrose and in cell cytoplasm...

In vivo and in vitro properties of the Transfer Factor (TF) and Migration Inhibition Factor (MIF) obtained from 2,4-dinitrofluorobenzene (2,4-DNFB) and tuberculin systems were studied simultaneously. Purified fractions of TF and MIF were obtained by Geon-Pevikon block electrophoresis and Sephadex G-200 exclusion chromatography. The physical and biological properties of these purified fractions ...

Pharmacological studies were done on the root bark of mulberry tree and pharmacological effects were compared with the clinical effects of "Sohakuhi" in Chinese medicine. n-Butanol- and water-soluble fractions of mulberry root had similar effects except for those on the cadiovascular system. Both fractions showed cathartic, analgesic, diuretic, antitussive, antiedema, sedative, anticonvulsant, ...

An extract of Smilax china, Hemidesmus indicus and Nigella Sativa on the ratio 3:2:1, prepared by boiling in water and concentrated could completely cure cases of oral canger diagnosed by modern methods. Cytotoxic studies with the three components showed activity in Nigella sativa at a concentration of 25 microgram equivalent of the dry powder against Dalton's lymphoma ascites cells. Animal exp...

Problem statement: Persea cordata previously demonstrated antispasmodic action against two neurotransmitters. It is important confirm such action against other neurotransmitters. Approach: Present study described the antispasmodic activity of polar (EtOAc; n-BuOH) and non polar (nhexane; dichloromethane) fractions from the bark of Persea cordata, against guinea pig ileum contracted by 5-hydroxy...

GUINEA PIGS SENSITIZED WITH TUBERCLE BACILLI DEMONSTRATE A DUAL ALLERGIC RESPONSE MEDIATED BY TWO CHEMICALLY DISTINCT PLASMA FRACTIONS: 1. Antibody to tuberculopolysaccharide is located exclusively in fraction II (gamma globulin). This fraction will passively transfer systemic anaphylaxis and urticarial type skin reactivity to tuberculopolysaccharide, and contains the Middlebrook-Dubos antibody...

Background: The constraints imposed by the pandemic COVID-19 increased risks of disruption supply chains, bringing new challenges to companies. These effects were felt more intensely in less-developed countries, which are highly dependent on imports products and raw materials. This study aims assess impact chain resilience a country (Guinea-Bissau) using complex adaptive system theory. Methods:...

In I9O 7, Sachs and Teruuchi 1 reported that if fresh guinea pig serum were appropriately diluted with distilled water (optimum relationship, I part serum to 8 to 9 parts distilled water), and incubated for an hour or more in a water bath at 37 ° C., on the restoration of isotonicity, complement activity no longer took place. The absence of hemolysis they attributed to a permanent' destruction ...