A valuable composition of Mentha piperita L. essential oil is menthol that is widely used fordifferent industries. The plants were treated with different concentrations of Methyl Jasmonate (MeJA) and after 24 h were evaluated for total soluble proteins, chlorophylls (a, b, and total), malondialdehyde, carbohydrates and antioxidant enzymes (Superoxide dismutase and Guaiacol peroxidase). Variance...

Guaiacol peroxidases (GP) are haem-containing enzymes participating in many physiological processes in plants. The expression pattern of these enzymes is organ-specific and developmentally regulated. Methods: The presence of GP activity in extract samples, prepared from Crocus sativus L. corms that were either dormant or rooting for 3, 6 and 10 days, was investigated. Results: Kinetic studies r...

In various models designed to imitate living cells the surface of the protoplasm is represented by guaiacol which acts in some respects like certain protoplasmic surfaces. The behavior of water in these models presents interesting features and if these occur in vivo, as appears possible, they may help to explain some of the puzzling aspects of water relations in the living organism. When suffic...

The use of soil additives such as selenium can positively influence the antioxidative system plants, making them more tolerant to abiotic stresses. aim this work was evaluate concentration sodium selenite or selenate applied substrate that causes improve in plant growth and enzyme activities sunflower plants. treatments were divided in: control (absence selenium); 0.2 mg L-1 selenate; 0.4 0.8 s...

The major pool of peroxidase activity is present in the peel of some Egyptian citrus species and cultivars compared to the juice and pulp. Citrus jambhiri cv. Adalia had the highest peroxidase activity among the examined species. Four anionic and one cationic peroxidase isoenzymes from C. jambhiri were detected using the purification procedure including ammonium sulfate precipitation, chromatog...

Although several investigations have demonstrated a plasma membrane (PM)-bound peroxidase activity in plants, this study is the first, to our knowledge, to purify and characterize the enzymes responsible. Proteins were extracted from highly enriched and thoroughly washed PMs. Washing and solubilization procedures indicated that the enzymes were tightly bound to the membrane. At least two distin...

The effect of heat treatment on polyphenoloxidases and peroxidase activities in three tissues (cambium, central cylinder internal bark) the varietie cassava roots (Manihot esculenta CRANTZ) Bonoua 2 were studied over a range 30 to 70 °C, using pyrogallol, 4-methylcatechol, dopamine pyrocatechol as substrates for polyphenol oxydase guaiacol substrate peroxidase. Optimal conditions enzym...

Sensitivity of common onion (Allium cepa L.) and faba bean (Vicia faba L.) to cadmium (Cd) stress was investigated using genotoxicity endpoints. Simultaneously, the antioxidative stress enzymes (guaiacol peroxidise and catalase) and lipid peroxidation [malonaldehyde (MDA) content] were investigated in the plants exposed to Cd in solution. The endpoints screened for genotoxicity included chromos...

The activities of polyphenol oxidase (PPO) and peroxidase (POD) in hot pepper (Capsicum annuum L.) pericarp were evaluated using spectrophotometric method. The enzymes were extracted from the pepper pericarp 5 g with 20 mL of 0.1 M phosphate buffer solution pH 7. PPO and POD activities were determined using catechol and guaiacol as a substrate, respectively. The effects of the amounts of enzyme...

AIMS To investigate the occurrence and distribution of thermo-acidophilic bacteria (TAB) associated with various commercial fruit crop soils in Japan and to assess their ability to produce the odorous phenolic compound, guaiacol. METHODS AND RESULTS Phylogenetic analysis based on the 5' end of the 16S rRNA gene (approximately 500 bp), was performed on 62 TAB isolated from the soil of several ...