A rat hepatoma cell line (Q7) of Morris hepatoma origin was transfected with a construct containing the tandem dimer genome of human hepatitis B virus (HBV) and the neomycin-resistant selection marker. The culture medium of several neomycin-resistant single-cell clones was found to accumulate high levels of secreted HBV surface antigen and core-related e antigen. HBV-specific replication interm...

OBJECTIVE To describe cancer incidence and mortality in Manizales during the 2003-2007 period from population-based information. METHODS The information was obtained from the Manizales Cancer Registry and DANE. We analyzed new cases and cancer deaths of individuals residing in Manizales from 1 January 2003 to 31 December 2007. Cases reported correspond to primary invasive malignant tumors, in...

OBJECTIVE Evaluate regional health care professionals' views of human papillomavirus (HPV) vaccination recommendations for adolescent patients through a mailed survey. METHODS A 16-question self-administered questionnaire was mailed to 518 physicians, physician assistants, and nurse practitioners in Dane County, Wis, working in family medicine, pediatrics, or gynecology in September 2006. The...

A mouse model of hepatitis B virus (HBV) infection produced by hydrodynamic injection of HBV DNA has been recently established. However, the ultrastructural demonstration of HBV particles in this mouse model has not as yet been reported. In our study, plasmid DNA containing wild-type HBV DNA was rapidly injected into 8-week-old female SCID mice via the tail vein. Serum levels of HBsAg were meas...

Hepatitis B e antigen (HBeAg) occurs in the serum of individuals infected with hepatitis B virus both free and in association with IgG. Utilizing a succession of steps involving salt precipitation, affinity chromatography, ion-exchange chromatography and isoelectrofocusing, we isolated free and IgG-bound forms of HBeAg from the sera of infected individuals with an overall gain in specific activ...

OBJECTIVE Comparing cervical cancer mortality rates in Colombian departments, as well as in urban and rural areas and examining the potential causes of any differences. METHODOLOGY This was an ecologic study. Mortality due to cervical cancer was estimated from data collected between 2005 and 2008 by the Colombian National Statistics Bureau (DANE).This included overall mortality in Colombia, m...

Verbindungen zwischen Mailservern über das Simple Mail Transfer Protocol (SMTP) können zur Sicherung des Übertragungskanals TLS-verschlüsselt werden. Die dafür verwendeten Zertifikate basieren auf CA-Vertrauen, sind oft nicht verifizierbar oder schlicht abgelaufen. Im Gegensatz zur TLS-Verschlüsselung im Browser kann hier keine manuelle Autorisierung durch einen Mail-Administrator erfolgen, son...