Recent data allow the assertion that conformational changes in F-actin regulate actin-myosin interaction in muscle fibre (Adelstein and Eisenberg 1980; Lednev 1980; Yanagida and Oosawa 1980; Borovikov et al. 1982; Borovikov and Gusev 1983 ; Borovikov and Karandashov 1983). However, the exact mechanism of such regulation cannot be established without further experimental evidence. The study of c...

BACKGROUND Occupational disease linked to the paper recycling industry has not been well documented. No previously confirmed formal diagnosis of occupational asthma (OA) caused by hydroxylamine has been made. METHODS We have assessed and performed occupational assessment of eight workers involved in this industry. Two of these were later diagnosed with OA and are reported here. RESULTS Both...

glutaraldehyde before density analysis in CsCl gradients, which gave a particle density of 1.38g/cm3, indicating the characteristic protein:RNA ratio (w/w) of 4:l of hnRNAprotein particles (Samarina et al., 1967). When isolated 3H-labelled particles were incubated at 37°C for 1 h, a shift to lower sedimentation values was observed. However, there was no detectable loss of 3H radioactivity from ...

In this investigation, an amino functionalized adsorbent was developed by grafting 4-aminobenzoic acid onto the backbone of cross-linked chitosan beads. The 3 sets of beads including chitosan (CX), glutaraldehyde cross-linked chitosan (CCX), and 4-aminobenzoic acid grafted cross-linked chitosan (FGCX) were characterized by FTIR, XRD, SEM, and TGA. The water content and amine concentration of FG...

To maintain optimum mechanical properties in glutaraldehyde-treated heart-valve tissue the full collagen crimp geometry originally present in the relaxed fresh tissue should be retained. By varying the pressure at which glutaraldehyde fixation is carried out, considerable alterations to this crimp geometry can be achieved. The mechanical stiffness of the preserved tissue is consequently affecte...

Eosinophil (and heterophil) leukocytes of glycogen-induced rabbit peritoneal exudates were fixed for 1(1/2) min in 2% glutaraldehyde and examined for acid phosphatase activity both biochemically and cytochemically. Biochemical assays showed that enzymatic activity had been inhibited by only approximately 10% under these conditions. The cytochemical reaction in the eosinophil was confined to the...

The effect of treatment of target tumor cells with glutaraldehyde (GA) on antibody-dependent macrophage-mediated cytolysis was investigated in a C3H/He mouse-MM46 syngeneic tumor system. GA-treated tumor cells were more susceptible to antibody-dependent macrophage-mediated cytolysis. Lysis of GA-treated cells was observed with less antibody and fewer macrophages than that of untreated cells. Ho...

Results from ultrastructural immunocytochemistry on glutaraldehyde-fixed cells confirmed and extended findings previously obtained with immunofluorescence. A microtubule-associated protein (MAP) of 210,000 molecular weight was shown to be specifically associated with all cytoplasmic and mitotic microtubules along their entire length in primate cells. Specific labeling with the anti-MAP antibody...

Industrial applications require enzymes highly stable and economically viable in terms of reusability. Enzyme immobilization is an exciting alternative to improve the stability of enzymatic processes. The immobilization of two commercial enzymes is reported here (cellulase and xylanase) using three chemical methods (adsorption, reticulation, and crosslinking-adsorption) and two polymeric suppor...

The rate at which the transmitter release machinery is fixed by 2% glutaraldehyde at hippocampal synapses and the amount of release evoked by the fixative have been investigated. We recorded from hippocampal cells while fixative was applied with a rapid flow system. Release is blocked in less than a second and fixative-produced exocytosis is at most a few percent of what would be caused by a hy...