A method for the purification of glutamate dehydrogenase from dogfish liver has been presented. The molecular weight of the enzyme has been determined and the sedimentation constant has been found to be invariant over a wide range of concentrations, in contrast to the bovine liver glutamate dehydrogenase. Upon electrophoresis the dogfish enzyme has been observed to migrate further than the beef...

Proline dehydrogenase (ProDH; 1.5.99.8) belongs to superfamily of amino acid dehydrogenase, which plays a significant role in the metabolic pathway from proline to glutamate. The goal of this research was gene cloning and characterization of ProDH enzyme from Pseudomonas fluorescens pf-5 strain. The gene encoding ProDH was isolated by means of PCR amplification and cloned in an IPTG inducible T...