1. The behaviour of rat liver alpha-glucosidases on dextran gel (Sephadex G-100) columns was studied. A ;retardation' of an acid alpha-glucosidase activity was observed. This activity was identified as lysosome alpha-(1-->4)-glucosidase. A single gel-filtration step resulted in a 700-fold purification of the enzyme. The same technique was also used to purify the acid alpha-glucosidase of human ...

BACKGROUND Lactose intolerance is a common health concern causing gastrointestinal symptoms and avoidance of dairy products by afflicted individuals. Since milk is a primary source of calcium and vitamin D, lactose intolerant individuals often obtain insufficient amounts of these nutrients which may lead to adverse health outcomes. Production of lactose-free milk can provide a solution to this ...

Glucosylceramide ,-glucosidase is a membrane-bound lysosomal hydrolase that is activated by acidic lipids, the most effective of which is phosphatidylserine (PtdSer), and an activator protein, saposin C. This report documents effects of Ca2+ ions on PtdSerand saposin C-enhanced ,-glucosidase activity. Ca2+ either increased or decreased enzyme activity, depending on (1) the concentration of phos...

Fruits of the plant Ficus Lacor Buch. Ham. were used traditionally for treatment diabetes mellitus. The present study was undertaken to evaluate antidiabetic potential using in vitro approach. Effect evaluated α/β -glucosidase, α-amylase and lipase enzyme inhibition assay methods. glucose absorption intestine everted rat jejunum while uptake isolated hemidiaphragm. Fruit cork ethanolic extract ...

Gaucher disease is caused by mutations in the gene encoding acid-beta-glucosidase. A recombinant form of this enzyme, Cerezyme, is used to treat Gaucher disease patients by ;enzyme-replacement therapy'. Crystals of Cerezyme after its partial deglycosylation were obtained earlier and the structure was solved to 2.0 A resolution [Dvir et al. (2003), EMBO Rep. 4, 704-709]. The crystal structure of...

β-glucosidase (E.C. 3.2.1.21), an enzyme involved in cellulose degradation, plays an important role in the soil organic carbon cycle. Cellulose is the most abundant organic compound in the biosphere so a product of its enzymatic hydrolysis is important as an energy source for soil microorganisms. Since β-glucosidase is very sensitive to different factors, determination of its activity might be ...

Sphagneticola calendulacea (L.) Pruski, a member of the Asteraceae family, is commonly found growing in India, Sri Lanka, China, and Southeast Asian countries. It has been utilized traditional medicine for treating various illnesses many nations. The objective this research was to compare phenolic content, antioxidant activity, inhibitory effect on - glucosidase S. leaf extracts obtained throu...

The β-glucosidase gene bgl3a from Myceliophthora thermophila, member of the fungal glycosyl hydrolase (GH) family 3, was cloned and expressed in Pichia pastoris. The mature β-glucosidase gene, which results after the excision of one intron and the secreting signal peptide, was placed under the control of the strong alcohol oxidase promoter (AOX1) in the plasmid pPICZαC. The recombinant enzyme (...

The gut regions of Epilachna chrysomelina are endowed with multiple enzymes that aid digestion host plant tissues. Digestive present in the E. during various developmental stages were studied laboratory to determine most destructive life stage beetle for proper management measures. Cellulase, α-glucosidase, amylase, lipase and proteinase activities observed guts. mid-gut recorded significantly ...