The poreplate sensilla of honeybees are equipped with multiple olfactory receptor neurons (ORNs), which innervate glomeruli of the antennal lobe (AL). We investigated the axonal projection pattern in glomeruli of the AL (glomerular pattern), formed by the multiple ORNs of individual poreplate sensilla. We used the different glomerular patterns to draw conclusions about the equipment of poreplat...

The vomeronasal system (VNS) mediates pheromonal communication in mammals. From the vomeronasal organ, two populations of sensory neurons, expressing either Gai2 or Gao proteins, send projections that end in glomeruli distributed either at the rostral or caudal half of the accessory olfactory bulb (AOB), respectively. Neurons at the AOB contact glomeruli of a single subpopulation. The dichotomi...

Olfactory glomeruli in insects share many features of organization with their vertebrate counterparts, and yet offer distinct advantages for study of neuronal development. Previous studies have revealed that the olfactory lobes of the brain of the moth Manduca sexta arise postembryonically and that glomeruli in the lobe are induced by olfactory afferent axons (Hildebrand et al., 1979; Oland and...

Recent investigation has indicated that TGF-beta1, the macrophage (Mphi) deactivator, may attenuate Mphi-mediated acute glomerular injury. Using stromelysin as an indicator, this study investigated whether and how endogenous TGF-beta1 modulates the glomerular cell activation triggered by Mphi. Rat mesangial cells were stably transfected with a cDNA encoding the active form of TGF-beta1 and a cD...

Micropuncture studies of acute renal failure after ischemic renal injury suggest that glomerular ultrafiltration coefficient may remain normal in the period immediately after ischemia and decline significantly during the following 18-24 hours. The present series of in vitro experiments was designed to evaluate glomerular ultrafiltration coefficient and glomerular oncometric and rheological prop...

Multiphoton microscopy (MPM) offers a unique approach for addressing both the function and structure of an organ in near-real time in the live animal. The method however is limited by the tissue-specific penetration depth of the excitation laser. In the kidney, structures in the range of 100 µm from the surface are accessible for MPM. This limitation of MPM aggravates the investigation of the f...

Nephrotic syndrome was experimentally induced by a single i.v. injection of puromyein. Permeability of the visceral epithelial wall of glomeruli was examined with electron microscope. 30-60 seconds prior to fixation, ferritin solution was injected into the urinary space of superficially located glomeruli either by direct micropuncture of the urinary space or by retrograde filling of the urinary...

Abstract All capillary endothelia, including those of the glomeruli, have a luminal cell surface layer (ESL) consisting glycoproteins, glycolipids, proteoglycans (PGs) and glycosaminoglycans. Previous results demonstrated that an intact ESL is necessary for normal filtration barrier damage to coupled proteinuria seen example in diabetic kidney disease (DKD). We used principles ion exchange chro...