The aim of the study was to validate tissue microarray (TMA) for vulvar cancer by comparing immunohistochemical staining results of triplicate core biopsies on TMA with the results of full section analysis. The study material consisted of slides and selected tissue blocks from 40 patients with vulvar cancer. A TMA was constructed with 3 cores/case. Both the TMA and the slides were stained with ...

Quantifying bacterial abundance and biomass is fundamental to many microbiological studies. Directly counting via epifluorescence microscopy has become the method of choice, especially for environmental samples, and conventional techniques require filtration of cells onto black polycarbonate membrane filters. We investigated the utility of instead capturing stained bacterial suspensions on bioa...

Background & Aims: The role of P53 and proliferating cell nuclear antigen (PCNA) genes in inflamed and non-inflamed odontogenic cysts is related to cell proliferation but it is unclear. The aim of this study was immunohistochemical evaluation of P53 and PCNA expression in inflamed and non-inflamed dentigerous cysts Methods: In the present study, 23 dentigerous cysts (12 inflamed and 11 non-infl...

A polymerase chain reaction (PCR) protocol for detecting IS6110 repetitive insertion sequence of Mycobacterium tuberculosis (MTB) was tested on archival Papanicolaou (Pap)-stained fine needle aspirated (FNA) smears from 24 patients with cervical tuberculous lymphadenopathy and 30 negative controls. The protocol involved protease digestion or phenolchloroform extraction, and simple or nested PCR...

Background The tumor microenvironment (TME) is composed of highly heterogeneous extracellular structures and cell types such as endothelial cells, immune fibroblasts that dynamically influence communicate with each other. constant interaction between a its plays critical role in cancer development progression can significantly affect tumor’s response to therapy capacity for multi-drug resistanc...

In digital histopathology traditionally prepared and stained slides are scanned in a dedicated scanner to produce extremely high resolution images.The resultant image fidelity is affected bymany variables including the staining processes, scanner design/setup, and ultimately the image display. Little or no routine quality control is applied at any of these stages and as a result widely varying ...

(atypical thymoma); and those showing total loss of the organotypical features of the thymus and displaying overt cytologic evidence of malignancy correspond to high-grade or poorly differentiated thymic epithelial neoplasms (also designated, by convention, thymic carcinoma) (2,4). The grading is based on a combination of architectural and cytological parameters as observed on routine microscop...

Enterobius vermicularis is a nematod of large intestine in man. Extra intestinal localization of this parasite is rare. A 60 year old man was admitted to the hospital with the clinical manifestation of meningitis. In laboratory examination of cerebrospinal fluid (C.S.F), the appearance was red, Albumin level was high and the glucose showed normal value. Haematoxylin and Eosin stained slides pre...