Circular DNAs are extra-chromosomal fragments that become circularized by genomic recombination events. We have recently shown that yeast LTR elements generate circular DNAs through recombination events between their flanking long terminal repeats (LTRs). Similarly, circular DNAs can be generated by recombination between LTRs residing at different genomic loci, in which case the circular DNA wi...

An efficient adaptor long-range PCR (ALR-PCR) procedure was developed to detect genomic rearrangements in high-plasticity genomic regions between closely related strains of bacteria. The method was precisely optimized using a combination of high-speed experimental steps for the chromosomal localization and elucidation of deletions, inversions, duplications, or inserted sequences within a clone-...

brucella transmission and epidemiology depend on infecting species and biovar. therefore, exact identification of the brucella is important to design correct control and treatment strategies. in this study, we examined presence of other brucellae in isfahan. one hundred twenty brucella isolates were collected and genomic dna was extracted from them. omp2a fragment of all isolates were amplified...

Background & Aims: Bordetella pertussis, the causative agent of whooping cough, continues to infect human hosts even in those populations where infants and children are routinely vaccinated. Causes of pertussis epidemiology are not fully identified unless strains of the pathogen are characterized by molecular means. Golbally, Multi Locus Variable Number of Tandem Repeats analysis (MLVA) has pro...

It is essential to identify and determine the properties of native plants as natural genetic resources. The present study was performed to identify the Mindium (Michauxi) laevigata species using molecular and biochemical procedures such as genomic DNA extraction, sequencing, and antioxidant capacity and protein content determination at both vegetative and generative phases in various parts of t...

Brucellosis is a zoonotic disease caused by bacteria of the genus Brucella The disease is endemic in many areas, causing chronic infections responsible for reproductive disorders in infected animals. Here, we present eight complete genome assemblies of eight Brucella abortus strains isolated from water buffaloes farmed in the Campania region.

A subunit vaccine candidate was produced from Brucella suis 145 (biovar 4; expressing both the A antigen of Brucella abortus and the M antigen of Brucella melitensis). The preparation consisted mostly of polysaccharide (PS; >90% [wt/wt]; both cell-associated PS and exo-PS were combined) and a small amount of protein (1 to 3%) with no apparent nucleic acids. Vaccinated mice were protected (these...

Brucellosis is a zoonotic disease with veterinary, public health, and economic implications. The study aimed to estimate the seroprevalence of Brucella spp. among ruminants in Rafha, Saudi Arabia during January October 2022 identify camel strains based on glycosyltransferase gene sequence. Sera (n=1012) were collected from non-vaccinated sheep, goats, camels cattle different sex, age breed rand...