Interleukin 2 (IL-2), produced with and without co-stimulation by the Burkitt's lymphoma line Daudi, was purified 37,000-fold to apparent homogeneity from lymphocyte conditioned medium by (NH4)2SO4 precipitation, DEAE-cellulose ion-exchange chromatography, gel filtration, and chromatography on blue agarose and on Procion-red agarose. The purified IL-2 showed a 10(6) U/mg protein sp act. IL-2 pr...

We determined the sequence of events and identified and quantitatively characterized the mobility of moving structures present during the early stages of fibrin-clot formation from the beginning of polymerization to the gel point. Three complementary techniques were used in parallel: spinning-disk confocal microscopy, transmission electron microscopy, and turbidity measurements. At the beginnin...

Based on measurements of the dynamic viscosity, a strategy is proposed to find an expression that relates the viscosity of an epoxy compound during curing, to the temperature, shear rate, and degree of reaction. It appeared that the dynamic viscosity is a unique function of the effective shear rate (i.e. the product of frequency and strain) over a wide range of frequencies and strains after bei...

Using deconvolution microscopy, we visualized in real time fibrin network formation in the hydrated state. Individual mobile fibers were observed before the gel point determined by eye. After gelation, an initial fibrin network was seen, which evolved over time by addition of new fibers and elongation and branching of others. Furthermore, some fibers in the network moved for a time. We quantifi...

The site specific endonuclease Bam HI which is composed of subunits of a molecular weight of 22 000 [1] can aggregate to complexes of a molecular weight of 360 000. It is an acidic protein with an isoelectric point at pH 5.3. Optimal activity is reached at 13 mM MgCl2. A very simple method is presented to determine kinetic constants of restriction enzymes directly from agarose gel photographs w...

We determined the sequence of events and identified and quantitatively characterized the mobility of moving structures present during the early stages of fibrinclot formation from the beginning of polymerization to the gel point. Three complementary techniques were used in parallel: spinning-disk confocal microscopy, transmission electron microscopy, and turbidity measurements. At the beginning...

The New Zealand Treasury and the Reserve Bank of New Zealand both maintain and use comprehensive macroeconomic models of the New Zealand economy: NZM and FPS respectively. In this paper, shocks are applied to the two models to illustrate and compare their dynamic properties. The most notable differences arise from their characterisations of the inflation process. In NZM, inflation is modelled a...

Introduction: Despite the fact that the clinical implementation of polymer gel dosimetry has been facilitated after the introduction of normoxic gels, there are still complications in its clinical routine use that are under investigation. In the current work, the feasibility of using a normoxic polymer gel dosimeter named MAGICA has been investigated for use in our clinical brachytherapy applic...

Two-dimensional gel electrophoresis (2D-GE) employs isoelectric focusing in the first dimension and a separation of the proteins according to their molecular weights in the second dimension. The gels are then commonly stained using silver stain to visualize the protein pattern. This is a tedious and time-consuming procedure. Here, we demonstrate a combination of two newly developed methods – OF...