Methyldopa-treated gamma globulin can be demonstrated serologically on either the red cell surface or on latex beads by the indirect antiglobulin reaction. The development of a positive antiglobulin reaction was related to methyldopa concentration and the length and temperature of incubation of methyldopa with protein and could be partially inhibited by the addition of albumin to the incubation...

The major storage protein fraction, globulin-1 protein, of French bean (Phaseolus vulgaris L.) was analyzed by two-dimensional electrophoresis. The protein pattern suggested a more complex system for globulin-1 protein than the model of three polypeptides, alpha, beta, and gamma, differing in molecular weight. Isoelectrofocusing analyses of the individual proteins showed that each exhibited cha...

Starch electrophoresis was used to separate allergic sera into electrophoretically distinct albumin, alpha-, beta-, and gamma-globulin fractions. The identity of the various fractions was established by free and paper electrophoresis. The gamma-globulin fractions were also characterized by ultracentrifugation. The serum skin-sensitizing antibody to ragweed pollen was shown to be associated with...

The cellulose ion exchangers (1) have proved to be useful chromatographic media for the serum proteins. In the fractionation of normal human serum on the anion exchanger, diethylaminoethyl (DEAE) cellulose, application of buffer gradients of increasing concentration and decreasing pH results in the appearance of components in the effluent fluid in order of increasing electrophoretic mobility (2...

We have studied the serum electrophoretic patterns in 25 patients with sarcoidosis. The electrophoretic serum analysis was made by the method of MacDonald et al,1 normal values in paper electrophoresis are presented in Table I. The diagnosis of sarcoidosis was established in each case by clinical and laboratory studies, the biopsy showing the usual microscopic picture of non-casting epitheloid ...

Resistance to Listeria monocytogenes was demonstrated in guinea pigs undergoing systemic delayed-type hypersensitivity reactions to bovine gamma globulin.