Histone modifications are involved in the regulation of many processes in eukaryotic development. In this work, we provide evidence that AtHDA7, a HISTONE DEACETYLASE (HDAC) of the Reduced Potassium Dependency3 (RPD3) superfamily, is crucial for female gametophyte development and embryogenesis in Arabidopsis (Arabidopsis thaliana). Silencing of AtHDA7 causes degeneration of micropylar nuclei at...

Functional analyses of the Arabidopsis genome require analysis of the gametophytic generation, since approximately 10% of the genes are expressed in the male gametophyte and approximately 9% in the female gametophyte. Here we describe the genetic and molecular characterization of 67 Ds insertion lines that show reduced transmission through the male gametophyte. About half of these mutations are...

Atallah, Nadia Ph.D., Purdue University, May 2015. Profiling Gene Expression During Early Gametophyte Development and Sex Determination in Ceratopteris Richardii. Major Professor: Jo Ann Banks. In the fern Ceratopteris richardii, every spore has the potential to develop as either a male or hermaphroditic gametophyte. Gametophyte sex is determined by a GA-like pheromone (ACE) that is secreted by...

Haploid Ceratopteris gametophytes are either hermaphroditic or male. The determinate of sex type is the pheromone antheridiogen (ACE) which is secreted by the meristic hermaphrodite and promotes ameristic male development of sexually undetermined gametophytes. Several mutations effecting the sex of the haploid gametophyte have been isolated and are described. The hermaphroditic (her) mutants ar...

On rocky shores, wave-swept macroalgae experience dramatic and repeated wave-induced hydrodynamic forces. However, previous studies of macroalgal mechanics have shown that individual waves are not forceful enough to account for observed rates of breakage. Instead, fatigue may contribute to algal breakage, with damage accumulating over time in conditions of repeated loading. Here I examine the e...

Reproductive traits in plants tend to evolve rapidly due to various causes that include plant-pollinator coevolution and pollen competition, but the genomic basis of reproductive trait evolution is still largely unknown. To characterize evolutionary patterns of genome wide gene expression in reproductive tissues in the gametophyte and to compare them to developmental stages of the sporophyte, w...

The chronic effect of dehydration on photochemical efficiency was determined in two heteromorphic life-history stages a cultivated red alga, Neopyropia yezoensis f. narawaensis (=Pyropia narawaensis; Bangiaceae, Bangiales) from Saga, Kyushu Island, Japan. response to acute emersion (~1440-min) under 50% humidity dissimilar between the stages, and effective quantum yields photosystem II microsco...

In Arabidopsis, a complex of Polycomb-group (PcG) proteins functions in the female gametophyte to control the initiation of seed development. Mutations in the PcG genes, including MEDEA (MEA) and FERTILIZATION-INDEPENDENT SEED 2 (FIS2), produce autonomous seeds where endosperm proliferation occurs in the absence of fertilization. By using a yeast two-hybrid screen, we identified MEA and a relat...

The administration of analogs, both agonists and for nonlethal population control of wildlife species. antagonists, of GnRH and immunization agalnst GnRH have Relatively little research has been done in this area. This been investigated for their ability to control reproductive chapter reviews relevant studies with domestic species and function in domestic species. These methods can be used dis...

In vitro gametogenesis raises new possibilities for reproductive and regenerative medicine as well as vexing policy challenges.