background: the study of the association between genotype and phenotype is of great importance for the prediction of many diseases and pathophysiological conditions. the relationship between angiotensin-converting enzyme (ace) gene insertion/ deletion (i/d) polymorphism and pathological processes such as coronary artery disease (cad) has been investigated previously with discordant results. thi...

background: the majority of α-thalassemi mutations are deletions of one or both α-globin genes. since the iranian populaion is a mixture of different ethnic groups, frequency and distribution of globin mutations in various regions of the country need to be clarified. the aim of this study was to determine the common alpha globin gene deletions among individuals with hypochromic microcytic anemi...

background ovarian cancer is the most common fatal gynecologic malignancy in women. the brca2 gene has a role in regulation of cell cycle during proliferation, differentiation and dna repair. changes in the methylation of brca2 may be an effective mechanism for ovarian cancer. objectives the aim of the present study was to evaluate the association between ovarian cancer and methylation status o...

The present study was carried out to genotypically characterize Staphylococcus aureus (S. aureus) isolated from bovine mastitis cases. A total of 37 strains of S. aureus were isolated during processing of 552 milk samples from 140 cows. The S. aureus strains were characterized phenotypically, and were further characterized genotypically by polymerase chain reaction using oligonucleotide primers...

Designing and producing a proper fusion construction is the most important problem of producing large quantities of a properly folded functional protein. This construction should have all necessary components of a real gene. A good designed fusion gene construction could be cloned into a good and suitable host. Clostridium perfringens is an important pathogen of humans and livestock and produce...

lactoferrin is a glycoprotein with molecular weight 80 kda iron-binding bond, which is composed of 690 amino acids. in most mammalian body fluids such as sweat, semen, tears, and saliva and milk neutrophil granules there. bovine lactoferrin gene be associated with susceptibility/resistance to mastitis and even with some economically important production traits. this study was carried out to det...

background: the aim of the study was to provide a point of reference to study the neospora caninum infections in china. methods: genome dna was extracted from the brains of aborted fetuses and specific pcr was performed with n. caninum nc5-targeted specific primers. fetal bovine brain tissues were homogenized and continuously cultured in vero cells with double antibodies. the medium was replace...

production of transgenic cotton (gossypium hirsutum l.) was studied utilizing agrobacterium-mediated strain lba4404 and pbi121 plasmid. this plasmid contains gus as a reporter gene driven by the camv35s promoter and nptii gene driven by nos as selection marker. in this study, the inheritance of gus gene was evaluated using selective media, pcr and gus staining in the second genertaion (t1) of t...