Selenium has cancer-preventive activity that is mediated, in part, through selenoproteins. The role of the 15-kDa selenoprotein (Sep15) in colon cancer was assessed by preparing and using mouse colon CT26 cells stably transfected with short hairpin RNA constructs targeting Sep15. Metabolic (75)Se labeling and Northern and Western blot analyses revealed that >90% of Sep15 was downregulated. Grow...

Exposure of cells to adenosine receptor (AR) agonists leads to receptor uncoupling from G proteins and downregulation of the A(1)AR. The receptor levels on the cell surface generally recover on withdrawal of the agonist, because of either translocation of the sequestered A(1)AR back to plasma membrane or de novo synthesis of A(1)AR. To examine the mechanism(s) underlying A(1)AR downregulation a...

Avila and Molina [1] introduced the notion of generalized weak structures which naturally generalize minimal structures, generalized topologies and weak structures and the structures α(g),π(g),σ(g) and β(g). This work is a further investigation of generalized weak structures due to Avila and Molina. Further we introduce the structures ro(g) and rc(g) and study the properties of the structures r...

We conducted the Resist Tubule space experiment to clarify role of cell wall in resistance inflorescence stems Arabidopsis (Arabidopsis thaliana) gravitational acceleration Cell Biology Experiment Facility on Kibo Module International Space Station. The rigidity was lower under microgravity conditions than at 1g, particularly basal region, which plays a central supporting entire plant presence ...

The G protein-coupled estrogen receptor GPER1/GPR30 is implicated in blood pressure regulation but the mechanisms are not identified. Here, we hypothesize that GPER1 controls blood pressure by regulating vascular smooth muscle cell Ca(2+) handling. Treatment with the GPER1 agonist G-1 (in the µM concentration range) acutely reduced spontaneous and synchronous Ca(2+) spike activity in A7r5 vascu...

The expression of the retinoic acid-induced G (Rig-G) gene, an all trans retinoic acid (ATRA)-inducible gene, was observed in multiple cancer cells, including lung cancer cells. However, whether Rig-G is a tumor suppressor in lung cancer is unknown. Here, we found that ectopic expression of Rig-G can lead to a significant decrease in proliferation of lung cancer cells, resulting in an inhibitio...

background: pentavalent antimonials are the first line drugs for the treatment of leishmaniasis. unresponsiveness of leishmania spp. to antimonial drugs is a serious problem in some endemic areas. investigations on molecular mechanisms involved in drug resistance are essential for monitoring and managing of the disease. cal-cineurin is an essential protein phosphatase for number of signal trans...

background: the aim of this study was to clarify the mechanism by which lactobacilli exert their cytotoxic effects on cervical cancer cells. in addition, we aimed to evaluate the effect of lactobacilli on the expression of human papilloma virus (hpv) oncogenes. materials and methods: in this experimental study, using quantitative real-time polymerase chain reaction (pcr), we analyzed the expres...

objective(s):our recent report indicates that breviscapine play a protective role of the kidney by down-regulating transforming growth factor-β1(tgf-β1), α-smooth muscle actin (α-sma) and alleviating interstitial fibrosis following unilateral ureteral obstruction (uuo). in this study, we investigate the effect of breviscapine on changes of renal water and sodium transport proteins in response t...