نتایج جستجو برای: functional assay
تعداد نتایج: 791404 فیلتر نتایج به سال:
The popular screening method for urotensin (UT) receptor antagonists is to measure the intracellular calcium concentration with a calcium-sensitive fluorescent dye. This assay format has an inherent limitation on the problem related to the fluorescence interference as it involves fluorescent dyes. In the present study, a label-free assay for the screening of UT receptor antagonists was develope...
Hfq is a bacterial master regulator which promotes the pairing of nucleic acids. Due to high molecular weight complexes formed between acids and amyloid form protein, it difficult analyze solely by gel shift assay formed, as they all migrate at same position in gel. In addition, precise kinetics measurements are not possible using assay. Here, we used synchrotron-based biophysical approach, syn...
Abstract Nanopores constitute an important class of biotechnologically relevant proteins. Unlike binders and enzymes, their experimental characterization is limited to high-resolution, yet low throughput biophysical methods. Addressing this technological gap, the functional nanopore (FuN) screen now provides a versatile assay study engineer nanopores in Escherichia coli combining quantitative r...
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