نتایج جستجو برای: functional assay

تعداد نتایج: 791404  

Journal: :Assay and drug development technologies 2014
Mi Young Lee Jihye Mun Jeong Hyun Lee Sunghou Lee Byung Ho Lee Kwang-Seok Oh

The popular screening method for urotensin (UT) receptor antagonists is to measure the intracellular calcium concentration with a calcium-sensitive fluorescent dye. This assay format has an inherent limitation on the problem related to the fluorescence interference as it involves fluorescent dyes. In the present study, a label-free assay for the screening of UT receptor antagonists was develope...

Journal: :Applied sciences 2022

Hfq is a bacterial master regulator which promotes the pairing of nucleic acids. Due to high molecular weight complexes formed between acids and amyloid form protein, it difficult analyze solely by gel shift assay formed, as they all migrate at same position in gel. In addition, precise kinetics measurements are not possible using assay. Here, we used synchrotron-based biophysical approach, syn...

Journal: :Biospektrum 2023

Abstract Nanopores constitute an important class of biotechnologically relevant proteins. Unlike binders and enzymes, their experimental characterization is limited to high-resolution, yet low throughput biophysical methods. Addressing this technological gap, the functional nanopore (FuN) screen now provides a versatile assay study engineer nanopores in Escherichia coli combining quantitative r...

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