introduction: β-globin gene cluster haplotypes are useful in diagnosis of particular molecular defects in β-thalassemia, prenatal diagnosis of β-thalassemia, and elucidating population affinities. methods: β-globin gene cluster haplotypes were studied in 150 β-thalassemia minor and 52 healthy in-dividuals from the fars province of iran. dna was extracted from leukocytes of whole blood by phe-no...

cryptosporidium is an apicomplexan parasite of humans and a wild range of domestic as well as wild animals. an 833-bp fragment of the 18s-rrna gene was used to identify cryptosporidium spp. recovered from children and adult patients, in human immunodeficiency virus (hiv) positive and negative patients in iran. initial identification of cryptosporidiosis was carried out by ziehl-neelsen acid-fas...

background:it has been hypothesized that genetic factors other than histocompatibility disparity may play a role in predisposition to developing chronic myelogenous leukemia (cml). in this regard, th1 and th2 cytokines and their gene polymorphism seems to be important. overall expression and secretion of cytokines is dependent, at least in part, on genetic polymorphism (nucleotide variations) w...

background: nowadays, oocytes and embryos vitrification has become a routine technique. based on clinical judgment, re-vitrification maybe required. but little is known about re-vitrification impact on genes expression. objective: the impact of re-vitrification on apoptotic and implanting genes, bax, bcl-2 and erbb4, at compaction stage embryos were evaluated in this study. materials and method...

introduction: hyperprolactinemia is a common serious side effect of antipsychotic medications that are currently used in the treatment of patients with schizophrenia. pharmacogenetic approaches offer the possibility of identifying patient-specific biomarkers for predicting the risk of this side effect. we investigated a possible relationship between variants (snps) in genes for cytochrome 2d6 (...

background: niche cells, regulating spermatogonial stem cells (sscs) fate are believed to have a reciprocal communication with sscs. the present study was conducted to evaluate the effect of ssc elimination on the gene expression of glial cell line-derived neurotrophic factor (gdnf), fibroblast growth factor 2 (fgf2) and kit ligand (kitlg), which are the main growth factors regulating sscs deve...

background: one of the most important producers of high quality industrial enzymes is the gram-positive bacterium, bacillus subtilis (b. subtilis). one major limitation that hinders the wide application of b. subtilis is the secretion of high levels of extracellular proteases which degrade the secreted foreign proteins. in this study, homologus recombination technique was used to knock out its ...

background: peroxisome proliferator activated receptor gamma (pparγ), a member of nuclear receptor superfamily, comprises two isoforms in mouse. these two isoforms are encoded by different mrnas, which are arisen by alternative promoter usage. there are two promoter regions upstream of pparγ gene. a 3 kb fragment, containing several transcription factor binding sites, acts as pparγ1 promoter re...

new cancer therapies with novel mechanisms and functions are needed to treat patients with different cancers. virotherapy is a good scenario for such treatment. the advantages of virotherapy include the potential lack of cross resistance with standard therapies and the ability to cause tumor destruction by numerous mechanisms. oncolytic virus not only possesses unique mechanisms of action that ...