The reproductive responses to one and two inseminations of frozen-thawed semen were compared in fat-tailed ewes which had previously been used in controlled reproduction programs aimed at estrus synchronization, multiple ovulation embryo transfer, and out of breeding season. In the trials, mature dry (n = 50) ewes in progestagen-induced estrus were inseminated 54 to 56 h after sponge removal. H...

The first pregnancy achieved in a seronegative woman following in-vitro fecundation through intracytoplasmic sperm (ICSI) injection from a man with autoimmune deficiency syndrome (AIDS; HIV-1 carrier) is reported. The semen was prepared by PureSperm and swim-up techniques. Some of the motile spermatozoa obtained were used to detect the presence of HIV-1 using the polymerase chain reaction techn...

BACKGROUND The effect of different thawing procedures for ram semen frozen in minitubes and mini straws on the fertility of sheep was tested in a field trial. METHODS Altogether, 719 Norwegian Crossbred ewes, aged between six months and six-and-a-half years from 8 farms, were inseminated vaginally in natural oestrus with frozen-thawed semen. Minitubes were thawed at 70 degrees C for 8 sec (T7...

The characteristics of male and female reproductive tracts and reproductive physiology in camelids are described. An account is given on methods of collection, characteristics and storage of semen, and fertility after artificial insemination (AI) with fresh, liquid-stored and frozen-thawed lamoid and camel semen.

The study had two objectives, namely, to estimate the andrological disorders leading to disposal of Karan Fries (KF), Sahiwal cattle and Murrah buffalo bulls and to study the effect of various factors (species/breeds, season of birth and period of birth) on male reproductive parameters. Records on occurrence of subfertility problems and disposal pattern of bulls maintained at the National Dairy...

This review describes the historical and current methods used for storage of bovine semen. The essential physiological differences between liquid and frozen semen, their relative advantages and disadvantages are addressed, and the current state of technology, the procedures used, their merits and future possibilities are also discussed.

Elevating the level of specific omega-3 fatty acids in equine semen by dietary modification can improve motion characteristics of cooled-stored and frozen-thawed sperm. Stallions, which produce semen that has poor tolerance to cooling and freezing, would benefit most from this dietary modification.

In an experiment involving the artificial insemination of 312 Merino ewes, fertilization, early (18 day), and late (100 day) pregnancy rates were examined after intrauterine insemination with frozen semen using a laparoscope. Oestrus was synchronized in ewes by progestagen sponge/PMSG treatment. After cervical insemination of fresh semen (control) 55 hrs after sponge removal, the fertilization,...

Effect of supplementation of ascorbic acid, caffeine and chloroquine diphosphate in the dilutor on post-thaw quality of spermatozoa was studied. Thirty-two semen ejaculates (8 each from 2 Holstein Friesian and 2 crossbred; 50% Friesian x 50% Haryana) were studied. Ascorbic acid, caffeine and chloroquine diphosphate were added in Tris-egg yolk-glycerol (control) extender at the concentration of ...

Semen was diluted 1:9 with egg yolk-citrate medium containing 0.31--3.1 M (final concentration) formamide, butyramide, acetamide, propionamide, dimethylformamide, lactamide, malomide, ethylene glycol, trimethylene glycol, dimethylsulphoxide (DMSO) or glycerol. After 30 min incubation at 20 degrees C, sperm motility was superior in hypertonic solutions of acetamide, lactamide, dimethylsulphoxid...