Background: Semen identification is assumed a crucial proof of sexual assault. Moreover, body fluids at the crime scene human being, such as blood, semen, and saliva, are often mixed. Methods: Hence, in our study, we aimed to use methylation analysis targeting DNA epigenetic markers Spectrin beta chain (B_SPTB_03) DEAD-box protein (DDX4) differentiate between fresh semen (less than 4 hours) age...

BACKGROUND Artificial insemination (AI) can serve as a powerful tool to the sheep owners for making rapid genetic progress of their flock. The AI in sheep is mostly performed using fresh semen with two reasons i) lambing rate following trans-cervical AI with frozen semen is limited by the inability of frozen-thawed sperm to transit the cervix and ii) the need of circumventing the cervical barri...

Aims: Determine the motility of spermatozoa in fresh goat semen after being given turmeric extract.
 Study Design: Randomized Block Design.
 Place and Duration Study: Sample: Galang, Deli Serdang Regency, Indonesia, between December 2022 January 2023.
 Methodology: Giving extract to once a day. The treatment included adding 0ml, 25 ml, 50 75 100 ml extract. parameter observed was...

PURPOSE Our aim was to evaluate the effect of neat semen vitrification on human sperm vital parameters and DNA integrity in men with normal and abnormal sperm parameters. MATERIALS AND METHODS Semen samples were 17 normozoospermic samples and 17 specimens with abnormal sperm parameters. Semen analysis was performed according to World Health Organization (WHO) criteria. Then, the smear was pro...

Sperm DNA damage affects the conception rate resulting from human assisted reproduction technology. The objective of this study was to adapt the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to provide a quality parameter for bull semen based on the detection of sperm DNA damage. Fresh semen was collected from two Japanese Black bulls (A, B) several times o...

The aim of this study was to see how chilled sperm quality is affected by ejaculation frequency and its correlation seminal plasma composition in INRA 180 rams. Five rams were collected at high (HFE) low frequencies (LFE). For the frequency, three times on same day every days for 18 days. In collection, consecutive ejaculates once a week four weeks. Ejaculates 20-minute intervals either HFE or ...

This study investigated two hypotheses: 1) that consistent between-boar variation in frozen semen quality exists and is genetically determined, and 2) that morphologically distinct subpopulations of spermatozoa exist within fresh boar ejaculates and that the incidence of these subpopulations is correlated with semen quality following cryopreservation. Five ejaculates were collected from each of...

In Artificial Insemination (AI) the semen is collected manually from a stud male and thereafter deposited (inseminated) in the female so that fertilization can occur in the absence of natural mating. Artificial Insemination, one of the earliest techniques for assisted reproduction in animals and humans, took longer to be implemented in dogs due to speciesspecific particularities. In past decade...

The most important aspect when discussing artificial ins realise that there are many factors that play a role in w bitch becomes pregnant after AI. These factors inclu (fresh vs frozen), semen quality and quantity, age and stud dog and bitch, site of semen deposition (intrauter time of insemination.... just to name a few! As you important that when comparing “conception” or more co rates” that ...

Artificial insemination (AI) is becoming more common in canine reproduction, permitting the use of shipped semen, assistance for geriatric or subfertile males, coverage of dominant females, and advanced reproductive technology such as intrauterine deposition of semen. AI may be performed with fresh, chilled, or frozen semen. All instruments should be clean and free of any chemical contamination...