The deterioration of rock mechanical properties under chemical-freezing-thawing-loading will seriously threaten the stability and safety engineering mass, so study its constitutive model has very important theoretical application significance. In view deficiency that existing statistical damage cannot describe nonlinear characteristics compaction stage post-peak shape, index is introduced to me...

BACKGROUND Spermatozoa cryopreservation is used for the management of infertility and some other medical conditions. The routinely applied cryopreservation technique depends on permeating cryoprotectants, whose toxic effects have raised the attention towards permeating cryoprotectants-free vitrification technique. OBJECTIVE To compare between the application of slow cryopreservation and vitri...

The relationship of contracture and exudation of water in frozenthawed frog muscle was studied. With maximum shortening, there was a water loss of 35 per cent of the weight of muscle. By restricting the contraction, it was demonstrated that the amount of water loss was proportional to the degree of shortening, there being no significant loss with isometric contraction. Muscle already shortened ...

Epididymal spermatozoa of domestic cats were diluted with TEST medium and frozen. The parameters - estimated percentage of motile spermatozoa, concentration of spermatozoa, cell morphology and transmigration rate (TMR) - were evaluated before freezing and after thawing. Transmigration is a new method to measure the percentage of spermatozoa that consistently move forward, and has not been inves...

Hemagglutinins for sheep red blood cells were detected in sera from JM virus-infected single-comb White Leghorn (susceptible S line) chickens. The agglutinins were not sedimented at 97,000 x g and were not affected by freezing and thawing, possibly indicating a soluble hemagglutinating factor. Agglutination titers were read in a relatively short period, after 4 hr of incubation at 4 C. The usef...

In 3D domain swapping, a domain of a protein breaks its noncovalent bonds with the protein core and its place is taken by the identical domain of another molecule, creating a strongly bound dimer or higher order oligomer. For some proteins, including diphtheria toxin, 3D domain swapping may affect protein function. To explore the molecular basis of 3D domain swapping in a well-characterized pro...

The purpose of the present study was to evaluate the effect of cryopreservation on 55 embryos which had one blastomere biopsied for preimplantation genetic diagnosis of aneuploidy before freezing. The thawing outcome was compared to that obtained in 94 embryos which derived from our conventional freezing programme in patients with comparable characteristics who were treated in the same period. ...

The sperm of each avian species and breed have unique characteristics that render them more or less susceptible to the freezing–thawing process; therefore, a suitable cryopreservation protocol is specific for type bird needed. In this context, little information about common pheasant’s available. Therefore, aim study was test different parameters at step process freezing into pellets thawing de...

Background To create hypothesis, which can give a logical explanation related to the benefits of freezing/thawing embryos. Cryopreservation is not only a technology used for storing embryos, but also a method of embryo treatment that can potentially improve the success rate in infertile couples. Methods From the analysis of multiple results in assisted reproductive technology, which have no s...