Typical microscopic methods used for characterizing intracellular protein mobility are, e.g., fluorescence photobleaching recovery (FRAP) and fluorescence correlation spectroscopy (FCS). Of these, FRAP can image protein mobility in entire two-dimensional sections of live cells, but is typically limited to the time resolution of confocal image series, some frames per second. FCS, on the other ha...

Antioxidant capacities of 56 selected Chinese medicinal plants were evaluated using the Trolox equivalent antioxidant capacity (TEAC) and ferric reducing antioxidant power (FRAP) assays, and their total phenolic content was measured by the Folin-Ciocalteu method. The strong correlation between TEAC value and FRAP value suggested that the antioxidants in these plants possess free radical scaveng...

Vasicine (1) was isolated from the ethanolic extract of Adhatoda vasica Nees (Acanthaceae) and the structure was confirmed using spectroscopic techniques. Acetylcholine esterase, trypsin, DPPH inhibition potential and FRAP assay were carried out using in vitro models. The results showed 38.4 ± 1.2% and 37.4 ± 1.1% activity in acetylcholine and trypsin inhibition assays respectively. The compoun...

Oxidative stress plays a major role in the pathogenesis of diabetic mellitus. The present study was undertaken to assess lipid peroxidation by TBARS, antioxidant status using FRAP and inflammatory status using sialic acid in patients with Type2 Diabetes mellitus and healthy individuals in rural population. AnthropometricBiochemical, Fasting insulin, HOMA-IR were estimated in both groups. We obs...

In vitro assays have contributed important insights into the mechanisms of RNA metabolism in cells. A growing collection of microscopy techniques is allowing the measurement of macromolecular binding and complex formation in the context of a real cell. We will first discuss two of the more established techniques. Fluorescence resonance energy transfer (FRET) identifies binding partners, pairs o...

This paper introduces the new fast response aerodynamic probe (FRAP®), which was recently developed at ETH Zurich. The provides time-resolved, three-dimensional flow measurements using virtual four sensor technique. Two probes work in tandem, being comparable to a pair of pneumatic needle probes. first probe, yaw angle sensitive, is positioned three circumferential positions. second pitch sensi...

The diffusion of fluoresceinated probes inside single collagen fibrils was investigated by imaging the migration of fluorescence along the fibrils in oil and by monitoring fluorescence recovery after photobleaching (FRAP). Probes were excluded from the fibrils according to their size. Probes that were not excluded diffused in the fibrils, but FRAP occurred 6 x 10(-4) times more slowly than in w...