We have developed a microneedle based system that is capable of extracting cell membrane lipids from real breast cancer tissues. We show that it is possible to extract phosphatidylcholine (PC) and phosphoethanolamine (PE) lipids from the cancer tissue samples with the microneedle system and analyse them with mass spectrometry (MS). PC and PE lipids act as biomarkers for breast cancer. In future...

Skin allografts only serve as temporary dressing for patients suffering major burns due to their high immunogenicity and rejection by the immune system, requiring systemic immunosuppressive therapies that lead deleterious side effects. Microneedle arrays composed of hyaluronic acid (HA) placed on skin can locally deliver immunomodulators simultaneously sample cells in interstitial fluid monitor...

To elucidate the molecular mechanism of muscle contraction resulting from the ATP-dependent actin-myosin interaction, we constructed an assay system with which both the force and the movement produced by the actin-myosin interaction in vitro can be simultaneously recorded and analyzed. The assay system consisted of the giant internodal cells of an alga, Nitellopsis obtusa, which contain well-or...

Received Feb 18, 2017 Revised Apr 20, 2017 Accepted May 10, 2017 In this study, a new, simple and cost-effective impedance detection of yeast cell concentration by using a novel integrated dual microneedle-microfluidic impedance flow cytometry was introduced. The reported method for impedance flow cytometry detection utilizes embedded electrode and probe in the microfluidic device to perform me...

Influenza virus-like particles (VLPs) are a promising cell culture-based vaccine, and the skin is considered an attractive immunization site. In this study, we examined the immunogenicity and protective efficacy of influenza VLPs (H1N1 A/PR/8/34) after skin vaccination using vaccine dried on solid microneedle arrays. Coating of microneedles with influenza VLPs using an unstabilized formulation ...

The clinical impact of biotechnology has been constrained by the limitations of traditional hypodermic injection of biopharmaceuticals. Microneedle patches have been proposed as a minimally invasive alternative. In this study, the translation of a dissolving microneedle patch designed for simple, painless self-administration of biopharmacetucials that generates no sharp biohazardous waste is as...

The aim of this project was to study the influence of microneedles on transdermal delivery of amantadine hydrochloride and pramipexole dihydrochloride across porcine ear skin in vitro. Microchannel visualization studies were carried out and characterization of the microchannel depth was performed using confocal laser scanning microscopy (CLSM) to demonstrate microchannel formation following mic...

This paper presents a developed process for fabrication of hollow silicon microneedle arrays. The inner hollow hole and the fluidic reservoir are fabricated in deep reactive ion etching. The profile of outside needles is achieved by the developed fabrication process, which combined isotropic etching and anisotropic etching with inductively coupled plasma (ICP) etcher. Using the combination of S...

J. Arya , Y-W. Chen , K. Dewitt , Y-W. Chiang , M. R. Prausnitz 1 1 Georgia Institute of Technology, 2 Merial Ltd. Purpose To stabilize rabies DNA vaccine in a dissolving microneedle patch and evaluate immunogenicity and dose sparing in dogs. Rabies is mainly a disease of animals, whereas humans get rabies primarily when they are bitten by dogs. Vaccinating domestic dogs and other animals has r...

patients with valvular heart disease and suffering atrial fibrillation of more than 12 months duration have a low probability of remaining in sinus rhythm after valve surgery alone. we performed intra-operative radiofrequency ablation or cryoablation as an alternative to surgical maze ііі procedure to create linear lesion lines for conversion of this arrhythmia to sinus rhythm. a total of 30 pa...