• Simple workflow and analysis—the simplicity and speed of PCR enables a rapid method for gene expression profiling with a ≤2-day workflow from sample to analysis. Intuitive bioinformatics through point-andclick run setup and automated secondary data analysis in Torrent SuiteTM Software with an associated plug-in, the AmpliSeqRNA, enables unambiguous gene expression analysis and visualization f...

Background: Scarring (cicatricial) alopecia represents a complex group of inflammatory disorders, mainly characterized by destruction of the hair follicle unit. Lichen planopilaris (LPP) and discoid lupus erythematosus (DLE) are the two main causes of primary cicatricial alopecia (PCA), both leading to hair follicle destruction and irreversible alopecia. However, they are different in pathogene...

Purpose. Evaluate if human papillomavirus (HPV) infection in lung cancer patients might be helping development by altering p16, p21, and PCNA, key genes involved cell proliferation tumor development. Methods. 63 fresh-frozen (FF) formalin-fixed paraffin-embedded (FFPE) samples from were used to detect HPV PCR, followed genotype through sequencing. The host gene expressions of PCNA quantified qP...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 206 No. 2 307–328 www.jcb.org/cgi/doi/10.1083/jcb.201403127 JCB 307 E. Frittoli and A. Palamidessi contributed equally to this paper. P.P. Di Fiore and G. Scita contributed equally to this paper. Correspondence to Pier Paolo Di Fiore: [email protected]; or Giorgio Scita: [email protected] Abbreviations used in this paper: CF...

Annotated formalin-fixed, paraffin-embedded (FFPE) tissue archives constitute a valuable resource for retrospective biomarker discovery. However, proteomic exploration of archival tissue is impeded by extensive formalin-induced covalent cross-linking. Robust methodology enabling proteomic profiling of archival resources is urgently needed. Recent work is beginning to support the feasibility of ...

The Informagen Next Generation Sequencing (NGS) Thyroid Test offers a model for the development of a multi-categorical assay for simultaneous interrogation of RNA and DNA markers. The Informagen test enables the in-depth molecular characterization of thyroid cancer fine-needle aspirate (FNA) and formalin-fixed paraffin-embedded (FFPE) specimens through NGS analysis of hotspot regions across a 1...

Hereditary breast cancer constitutes only 5-10% of all breast cancer cases and is characterized by strong family history of breast and/or other associated cancer types. Only ~ 25% of hereditary breast cancer cases carry a mutation in BRCA1 or BRCA2 gene, while mutations in other rare high and moderate-risk genes and common low penetrance variants may account for additional 20% of the cases. Thu...