We present measurements of collisional fluorescence quenching cross sections of NO(A(2)Σ(+), v' = 0) by NO(X(2)Π) and O2 between 34 and 109 K using a pulsed converging-diverging nozzle gas expansion, extending the temperature range of previous measurements. The thermally averaged fluorescence quenching cross sections for both species show a monotonic increase as temperature decreases in this te...

Fluorescently labeled oligonucleotide probes have been widely used in biotechnology, and fluorescence quenching by the interaction between the dyes and a nucleobase has been pointed out. This quenching causes big problem in analytical methods, but is useful in some other cases. Therefore, it is necessary to estimate the fluorescence quenching intensity under various conditions. We focused on th...

We have previously shown that in the red alga Rhodella violacea, exposure to continuous low intensities of light 2 (green light) or near-saturating intensities of white light induces a DeltapH-dependent PSII fluorescence quenching. In this article we further characterize this fluorescence quenching by using white, saturating, multiturnover pulses. Even though the pulses are necessary to induce ...

The interaction of losartan potassium (LP) with bovine serum albumin (BSA) was studied by fluorescence quenching in combination with UV–Vis spectroscopic method under near physiological conditions. The fluorescence quenching rate constants and binding constants for BSA–LP system were determined at different temperatures. The fluorescence quenching of BSA by LP is due to static quenching and ene...

The wavelength dependent fluorescence decay properties of bovine prothrombin fragment 1 have been investigated employing a picosecond time-correlated single photon counting technique. All observations are discussed with using the crystal structure (Soriano-Garcia et al., Biochemistry 31:2554-2566, 1992). Fluorescence lifetimes distribution and conventional multiexponential analysis, as well as ...

.a simple spectrofluorimetric method is described for the determination of doxorubicin (dxr) based on its quenching effect on the fluorescence intensity of tb 3+ - deferasirox (dfx) complex as a fluorescent probe. the excitation and emission wavelengths were 328 and 545 nm, respectively. the effects of ph, time, order of addition of reagents, concentrations of tb 3+ and dfx and the buffer volum...

As a typical biomineral, hydroxyapatite (HAp) is widely applied in bone implants and other related fields. However, the inherent nature of HAp can potentially be altered through restricting its fabrication conditions. Here, HAp fabricated by a hydrothermal treatment of pieces of natural seashell is demonstrated to have the capability of fluorescence quenching. To the best of the author's knowle...

The aim of this work was to study the interaction of sulpiride with human serum albumin (HSA) and bovine serum albumin (BSA) through the fluorescence quenching technique. As sulpiride molecules emit fluorescence, we have developed a simple mathematical model to discriminate the quencher fluorescence from the albumin fluorescence in the solution where they interact. Sulpiride is an antipsychotic...

The synthesis of a range of novel 3-hydroxypyridin-4-ones and 3-hydroxypyran-4-ones linked with different coumarin substituents is described. These compounds have been developed in order to provide a series of molecular probes for the quantification of intracellular labile iron pools. An evaluation of the effect of iron(III) on fluorescence intensity was undertaken. Chelation of iron(III) cause...