Burkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients. The aim of this work was to perform pulsed-field gel electrophoresis-based molecular typing of B. cenocepacia isolates to evaluate the epidem...

FACTORS THAT AFFECT PFG SEPARATIONS........................................................................ 294 Molecular Properties ............................................... .................................... ............. 294 Pulse Times............................................................................................................... 295 Electrical Field Shape ..............