A dynamic model of glucose overflow metabolism in batch and fed-batch cultivations of Escherichia coli W3110 under fully aerobic conditions is presented. Simulation based on the model describes cell growth, respiration, and acetate formation as well as acetate reconsumption during batch cultures, the transition of batch to fed-batch culture, and fed-batch cultures. E. coli excreted acetate only...

BACKGROUND Fungal morphology and aeration play a significant role in the growth process of Mortierella alpina. The production of microbial oil rich in arachidonic acid (ARA) in M. alpina was enhanced by using a multi-stage fermentation strategy which combined fed-batch culture with precise control of aeration and agitation rates at proper times. RESULTS The fermentation period was divided int...

[This corrects the article DOI: 10.1186/s13068-016-0466-5.].

Apple pomace extract (APE) in aerobic variable fed batch culture under standardized fermentation parameters was evaluated for the production of baker’s yeast. The substrate was fed at a flow rate of 0.39 dm h to regulate fermentable sugar concentration between 1-2 % in bioreactor. An average specific growth rate of 0.24 h and cellular yield coefficient of 0.48 g/g sugar was obtained during bake...

Recombinant Escherichia coli XL1-Blue harboring a high-copy-number plasmid containing the Alcaligenes eutrophus polyhydroxyalkanoate synthesis genes could efficiently synthesize poly(3-hydroxybutyrate) (PHB) in a complex medium containing yeast extract and tryptone but not in a defined medium. One of the reasons for the reduced PHB production in a defined medium was thought to be severe filamen...

A marked enhancement in the production of cell-bound phytase in Pichia anomala was achieved in cyclic fed batch fermentation (47333 UL -1 ) in comparison with that in batch (11569 UL -1 ) and fixed volume fed batch (36911 UL -1 ) fermentations in cane molasses medium. Phytase productivity was sustainable in cyclic fed batch fermentation over seven days, and therefore, this mode of fed batch fer...

The main aim of this work was to evaluate the efficiency producing caproic acid and other volatile fatty acids using a co-digestion between cheese whey sewage sludge in continuous reactor. effect two different feeding regimes (one per day) three hydraulic retention times (HRT) (15, 10 6 days) on organic production were studied. optimal conditions for process days HRT, 2 cycles day, reaching max...

Competitive exclusion of Salmonella enterica serovar Enteritidis by a mixed culture of Lactobacillus crispatus and Clostridium lactatifermentans was studied in a sequencing fed-batch reactor mimicking the cecal ecophysiology of broiler chickens. Growth of serovar Enteritidis was inhibited by a mixed culture of L. crispatus and C. lactatifermentans at pH 5.8 but not by a monoculture of L. crispa...

The purpose of this paper is to optimize the function of a batch reactor operating in open and closed loops. A simultaneous optimization method is employed. This optimization method provides optimal solution at a finite number of points in time. However, the actual batch reactor operation may deteriorate if the computed optimal input is improperly applied to the process. In the open-loop operat...

Biological hydrogen production by anaerobic bacterium, Rhodospirillum rubrum was studied in batch and continuous bioreactors using synthesis gas (CO) as substrate. The systems were operated at ambient temperature and pressure. Correlations available in the literature were used to estimate the gas-liquid mass transfer coefficients (KLa) in batch reactor. Based on experimental results for the con...