background: the occurrence of giardia duodenalis in cats is of potential significance from both clinical and public health perspectives. the object of this study was antigenic detection of g. duo­denalis in house­hold cats of ahvaz district, south-west of iran. methods: the prevalence of g. duodenalis was determined in fecal samples by two techniques: centrifuga­tion-flotation and a commercial ...

Three commercially available assays, designed to specifically detect the presence of Mycobacterium avium subsp. paratuberculosis (MAP) in fecal samples by IS900-PCR, were compared with a conventional culture method. Fecal samples from 100 dairy cows were tested. Fifty-four (67.5%) of 80 culture-positive samples were positive for an assay that detects MAP DNA by dot spot hybridization of polymer...

MicroRNAs (miRNAs) are known as small non-coding RNA molecules and down-regulator for specific mRNA expressions. In the oncology area, miRNAs play important roles such as carcinogenesis, invasion, and metastasis. To date, several studies have clarified that circulating miRNA in plasma (serum) was a potential marker for colorectal cancer detection [1,2], and was remarkably stable in plasma due t...

Fecal samples were collected from 4,361 dairy cows on 91 dairy operations between 26 February and 8 July 1996. Fecal samples were cultured for Escherichia coli O157, and positive isolates were probed for verotoxin-producing genes. A total of 52 (1.2%) fecal samples on 22 (24.2%) operations were positive for verotoxin-producing E. coli O157. Herds in which samples were collected on or after 1 Ma...

Spoilage of meat has remained a serious challenge in developing countries. The objective of this study was to evaluate the beef and lamb meat quality from supermarkets and shops in summer and winter season. Beef and lamb meat were stored at 4±1 o C during 5 days and test for microbiological properties, oxidation stability, and color properties, in beef and lamb meat. Samples were collected from...

The DNA of Cryptosporidium Oocysts was extracted from fecal samples by using Commercial Wizard Genomic DNA purification general kit, tow freezingthawing protocols have been compared in this study: deep freezer for three cycles and liquid nitrogen for fifteen cycles, the results indicate that the second protocol was more influential in obtaining a sufficient amount of DNA from Oocysts of Cryptos...

Nonparenteral transmission might contribute to human parvovirus 4 (PARV4) infections in sub-Saharan Africa. PARV4 DNA was detected in 8 (0.83%) of 961 nasal samples and 5 (0.53%) of 943 fecal samples from 1,904 children in Ghana. Virus concentrations ≤ 6-7 log(10) copies/mL suggest respiratory or fecal-oral modes of PARV4 transmission.

Two frequently applied genetic Bacteroidetes markers for total fecal pollution (AllBac and BacUni) were found in high numbers in pristine soil samples of two alpine catchment areas casting doubt on their value as fecal indicators. This finding underlines the necessity to evaluate assays locally and against non-intestinal samples before application.

Antibody detection-based tests for paratuberculosis offer speed and economy, 2 diagnostic test attributes important to animal industries with narrow profit margins. Application of such tests to individual milk samples instead of serum samples can further improve testing efficiency and decrease testing cost. Accuracy of a commercial bovine paratuberculosis enzyme-linked immunosorbent assay (ELIS...

A duplex PCR (D-PCR) amplifying portions of the Brachyspira hyodysenteriae NADH oxidase gene and the B. pilosicoli 16S rRNA gene was developed and then tested on DNA extracted from 178 porcine fecal samples. The feces also underwent anaerobic culture and species-specific PCRs. Fecal extraction-D-PCR detected seven additional samples containing B. hyodysenteriae and five more containing B. pilos...