word count: 221 / 250 max. Introduction word count: 644 / 750 max. Discussion word count: 1500 / 1500 max. Abbreviations P450, cytochrome P450; HLM, human liver microsomes; HPLC, high-performance liquid chromatography; IC50, inhibitor concentration producing 50% inhibition; kobs, inactivation rate constant, KI, inactivation binding constant; kinact, maximum inactivation rate constant; LC-MS, li...

A study has been made of the extraction of trans-resveratrol from grapes using pressurised liquids (PLE); for this, the first stage was to determine the stability of this compound during extractions at different temperatures (50, 100, 150 degrees C), with quantitative recoveries being obtained up to 150 degrees C. By employing solid-phase extraction (SPE) it was possible to retain this compound...

A h igh-per formance l i q u i d chromatography (HPLC) method has been deve loped f o r t h e s e p a r a t i o n of o l e f i n , s a t u r a t e , and a r o m a t i c hydrocarbons found i n h i g h b o i l i n g d i s t i l l a t e s and r e s i d u e s of s h a l e o i l and i n whole s h a l e o i l s . The dual-column chromatographic system uses s i l i c a g e l i n one column and s i l i...

Wavelength-routed all-optical networks have been receiving signiicant attention for high-capacity transport applications. A good routing and wavelength assignment (RWA) algorithm is critically important to improve the performance of wavelength-routed WDM networks. We study the blocking performance of xed-paths least-congestion (FPLC) routing in multiiber WDM networks in this paper. A new analyt...

gamma-aminobutyric acid is the main inhibitory amino acid neurotransmitter in several areas of the brain. measurement of gamma-aminobutyric acid in the brain will help elucidate its metabolic rote and diagnostic value. the liquid chromatography (lc) system consisted of a delivery pump. male rats (150-250g) were used in this study. the brain was rapidly removed from decapitated animals. trie chr...

Submit Manuscript | http://medcraveonline.com Abbreviations: NMR: Nuclear Magnetic Resonance; HPLC: High Performance Liquid Chromatography; RP-HPLC: Reverse Phase High Performance Liquid Chromatography; HPTLC: High Performance Thin Liquid Chromatography; TLC: Thin Liquid Chromatography; API’s: Active Pharmaceutical Ingredient’s; LOD: Limit of Detection; LOQ: Limit of Quantitation; FTIR: Fourier...

background and objectives:  high concentration of preservatives in food may result in gastrointestinal disturbances whereby some patients suffering from asthma, rhinitis, or urticaria. the aim of this study is the introduction and optimization a new method for simultaneous determination of four preservatives (sb, ps, mp, pp) in foodstuff by high performance liquid chromatography. materials and ...

Hybridoma clone C3A8 was established as a result of fusion between the lymphocytes of Balb/c mice immunized with the MCF-7 breast carcinoma cell line and Sp2/0-Ag14 myeloma cells. The clone was secreted the monoclonal antibodies (Mab) either in culture supernatant or ascites fluid and still have a contaminants which need to be purify in order to get the desired antibody. The main objective of t...

BACKGROUND We investigated whether ghrelin is present in human saliva, is produced by salivary glands, and physiologic consequences of these findings. METHODS Expression of ghrelin and specific receptor mRNA was determined by PCR. Proteins were identified by immunoblotting and size-exclusion fast protein liquid chromatography (FPLC) with consecutive RIA. Specific RIAs were used for quantifica...