Background and Objective: Tuberculosis is still a major health problem, involving about 1/3 of the world ́s population. Diagnosis is difficult when we only use Ziehl-Neelson staining. Many cases may be missed. A more rapid and sensitive diagnostic method is necessary. PCR may be helpful. The aim of this study is to compare PCR, Zieh-Neelsen staining and histopathologic findings in diagnosis of t...

Clostridium cellulolyticum H10 (ATCC 35319) has the ability to ferment cellulosic substrates into ethanol and weak acids. The growth alcohol production rates of wild-type organism are low and, therefore, targets metabolic engineering. A genomic DNA expression library was produced by a novel application degenerate oligonucleotide primed PCR (DOP-PCR) serially enriched in C. grown on cellobiose e...

The polymerase chain reaction (PCR) has been an essential tool for diagnosis of infectious diseases, but conventional PCR still has some limitations with respect to applications to point-of-care (POC) diagnostic systems that require rapid detection and miniaturization. Here we report a light-based PCR method, termed as photo-PCR, which enables rapid detection of bacteria in a single step. In th...

case: isolated talus osteomyelitis caused by koch's bacillus (bk) is extremely rare. in this paper we report an isolated talus bone tuberculosis in a 52 year-old man who presented a 2-month history of swelling, pain and functional disability of his right ankle. surgical bone curetting and histological examination showed granuloma with central caseating necrosis. acid fast stain and pcr exa...

In the era of the Human Genome Project, quantitation of gene expression by tumor/host cells is of paramount importance to investigate gene patterns responsible for cancer development, progression and response/resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstr...