Objectives. The aim of this study was to create a Czech translation the Perceived Stress Scale (PSS), assess its psychometric properties on representative sample general adult population, and compare original 14-item version (PSS-14) with shortened 10-item (PSS-10) four-item (PSS-4) versions. Sample setting. Two pilot studies were conducted final scale (n = 365 n 420). PSS administered populati...

The transcription factor, forkhead in rhabdomyosarcoma (FKHR), is phosphorylated at three amino acid residues (Thr-24, Ser-256 and Ser-319) by protein kinase B (PKB)alpha. In the present study, mutagenesis has been used to study the roles of these phosphorylation events in regulating FKHR function in transfected HEK-293 cells. We find that the overexpression of FKHR[S256A] (where Ser-256-->Ala)...

14.1 Members Kenji Ono (Team Leader) Jorji Nonaka (Researcher) Mikio Iizuka (Researcher) Chongke Bi (Postdoctoral Researcher) Daisuke Sakurai (Postdoctoral Researcher) Kazunori Mikami (Technical Staff) Tomohiro Kawanabe (Technical Staff) Seiji Fujino (Senior Visiting Scientist) Naohisa Sakamoto (Visiting Scientist) Masahiro Fujita (Visiting Technician) Kentaro Oku (Visiting Technician) Steve Pe...

Cyclin Y is a highly conserved cyclin among eumetazoans, yet its function and regulation are poorly understood. To search for Cyclin Y-interacting proteins, we screened a yeast two-hybrid library using human Cyclin Y (CCNY) as a bait and identified the following interactors: CDK14 and four members of the 14-3-3 family (ε, β, η, τ). The interaction between CCNY and 14-3-3 proteins was confirmed ...