BACKGROUND This study aims to analyze the active pharmaceutical ingredients (APIs) of licorice (Radix Glycyrrhizae; gancao), including glycyrrhizic acid, liquiritin, isoliquiritin and total flavonoids, in multi-ingredient and multi-phase extraction by online near-infrared technology with fiber optic probes and chemometric analysis. METHODS High-performance liquid chromatography and ultraviole...

OBJECTIVE The aim was to establish a method for measuring organ blood flow in rats using commercially available, coloured, dye extraction microspheres. METHODS A mixture of radiolabelled and dye extraction microspheres was infused into rats at rest (basal) and during intravenous administration of either angiotensin II (0.5 microgram.kg-1.min-1) or isoprenaline [12.5 ng.(g0.74)-1.min-1]. Tissu...

Cancer biomarker studies often require nucleic acid extraction from limited amounts of formalin-fixed, paraffin-embedded (FFPE) tissues, such as histologic sections or needle cores. A major challenge is low quantity and quality of extracted nucleic acids, which can limit our ability to perform genetic analyses, and have a significant influence on overall study design. This study was aimed at id...

Five institutional partners participated in an interlaboratory comparison of nucleic acid extraction, RNA preservation and quantitative Real-Time PCR (qPCR) based assays for biogas biocenoses derived from different grass silage digesting laboratory and pilot scale fermenters. A kit format DNA extraction system based on physical and chemical lysis with excellent extraction efficiency yielded hig...

Aloe greatheadii var. davyana (Asphodelaceae) is used among rural South African communities to treat arthritis, skin cancer, burns, eczema, psoriasis, digestive problems, high blood pressure and diabetes, despite very little supporting scientific evidence. Due to increased interest by both the scientific community and industry regarding the medicinal uses of this plant species, we identified, q...

Large amounts of the major storage proteins, beta-conglycinin and glycinin, in soybean (Glycine max) seeds hinder the isolation and characterization of less abundant seed proteins. We investigated whether isopropanol extraction could facilitate resolution of the low abundant proteins, different from the main storage protein fractions, in one-dimensional polyacrylamide gel electrophoresis (1D-PA...

Akar nipah (Nypa fruticans Wurmb) contains active chemicals in the form of flavonoids and is a component plant with antioxidant activity. The study's goal was to determine total flavonoid content as well activity akar extract. extraction method maceration methanol solvent. Total levels extract were determined using UV-Vis spectrophotometry after addition 10% AlCl 3 at wavelength 433 nm, while D...

The separation of liver ribonucleic acid and deoxyribonucleic acid from the associated protein material without hydrolysis can be accomplished by a salt-extraction procedure described by Barnum & Huseby (1950). This method, based on ribose recovery (Lowe & Rand, 1956; C. U. Lowe & P. R. Venkataraman, in preparation), yields 80% of the total ribonucleic acid of normal liver homogenate or cell fr...

OBJECTIVES An in vitro screening test was used to compare the cytotoxicity and elemental release from mercury- and gallium-based dental restorative materials. METHODS The test employed three sequential extractions of the samples into cell-culture medium which were then used to evaluate the cytotoxicity of the samples and the release of elements from the samples. Cytotoxicity was measured by p...

Introduction The identification and quantification of hexavalent chromium in solid samples is an important factor in characterization studies. The speciation of elements is a developing feature of analytical methods; the evaluation of the environmental and biological impact of the Cr (VI) compounds on environmental matrices is fundamental because of their demonstrated toxicity and potential car...