the aim of this study was to develop an easy analytical method for determining flam prop-m- iso propyl residues in honey by high performance liquid chromatography with uv detection (hplc/uv) for routine analysis. chromatographic separation was carried out at room temperature on a zorbax eclipse xdb- c18, 100 å pore size, 5 μm particle size (300 mm × 3 mm i.d.) column using a mobile phase of met...

This licentiate thesis is based on two papers that describe two original methods for analysing environmental pollutants. In both cases samples were extracted by onor off-line Matrix Solid Phase Dispersion (MSPD), then target analytes were separated from potentially interfering matrix components and analysed by Liquid Chromatography–Mass Spectrometry (LC-MS). Hot water and a small volume of meth...

background: taxus species are important due to their content of taxol, baccatin iii, 10- deacetyl baccatin and other taxoids suitable to change to active pharmaceutical ingredients, which are used as anti-neoplastic agents. investigations on taxoids carried out during the past four decades, resulted in identification of more than 350 taxoids. objective: this study aimed to isolate and identify ...

Introduction:Holmium-166 radionuclide is one of the most effective radionuclides used for targeted therapy with theranostic properties. One method to produce this radioisotope is via the decay of its parent (indirect method). In this study applicability of extraction chromatography (EXC) for separation of no carrier added 166Ho from neutron-irradiated natura...

This paper describes a new, highly sensitive, rapid method for the determination of low levels of endogenous melatonin, the pineal and retinal hormone, in fish plasma samples. The combination of solid-phase extraction and high-performance liquid chromatography with fluorescence detection significantly improves the separation and increases the sensitivity of the assay. The proposed method may be...

A new technique for separation and dctermination of ATP, ADP, AMP, NAD', NADP', NADH and NADPH in Lactobacillus plantarum has been developped. It involves acid and basic extraction of nucleotide pool, analysis by reverse-phase high performance liquid chromatography on a 5 p Spherisorb ODS-1 column and UV detection. The method offers advantages in accurate estimations of adenylic and nicotinamid...

We describe a routine method for determining plasma concentrations of quinidine by liquid chromatography. The procedure requires 1.0 ml of plasma (or serum) and involves internal standard addition, extraction with ether, and separation on a column of microparticulate silica. Day-to-day CV (15 days) was less than 5% and no deterioration in column performance has been observed during 12 months. C...

Sulfonamides were extracted from honey with acetate buffer (pH 5.0), and cleaned up by solid phase extraction (SPE) procedure. The liquid chromatography (LC) separation was carried out on RP C18 column using acetic acid and acetonitrile as the mobile phase in gradient mode and monitored with fluorescence detector, after pre-column derivatisation. Recoveries from spiked honey were above 80%, and...

Recently, ionic liquids (ILs) have gained in popularity as unique solvents in different areas of separation techniques. Owing to tunable properties which can be selected by choosing appropriate cationic or anionic constituents, they can be applied in chromatographic and electro-chromatographic methods as mobile phase or stationary phase modifiers or in sample preparations processes as new extra...

The example of column chromatography (Figure 1) demonstrates the typical features found in this analytical technique. The diagram shows an experiment where a two-component mixture is subjected to column chromatography. The column is packed with a solid material called the stationary phase. A liquid solvent or eluting solution is poured into the column and completely wets the solid packing mater...