The green alga Chlamydomonas reinhardtii elaborates two biochemically and morphologically distinct cell walls during its life cycle: one surrounds the vegetative and gametic cell and the other encompasses the zygote. Hydroxyproline-rich glycoproteins (HRGPs) constitute a major component of both walls. We describe the isolation and characterization of a zygote-specific gene encoding a wall HRGP....

Screening of 10 000 Arabidopsis transgenic lines carrying a gene-trap (GUS) construct has been undertaken to identify markers of seed germination. One of these lines showed GUS activity restricted to the endosperm, at the micropylar end of the germinating seed. The genomic DNA flanking the T-DNA insert was cloned by walking PCR and the insertion was shown to be located 70 bp upstream of a 2285 ...

A series of gene constructs encoding synthetic glycomodule peptides with N-terminal signal sequences and C-terminal green fluorescent proteins were expressed in transgenic Arabidopsis (Arabidopsis thaliana) under the control of the 35S promoter. The synthetic glycomodule peptides were composed of repetitive proline-containing motifs that have been previously found to be substrates for prolyl hy...

59:709-717. 2.Boshart, M., M. Kluppel, A. Schmidt, G. Schutz and B. Luckow. 1992. Reporter constructs with low background activity utilizing the cat gene. Gene 110:129-130. 3.Galas, D.J. and A. Schmitz. 1978. DNase footprinting: a simple method for the detection of protein-DNA binding specificity. Nucleic Acids Res. 5:3157-3170. 4.Hemsley, A., N. Arnheim, M.D. Toney, G. Cortopassi and D.J. Gala...

A cDNA encoding a putative lectin expressed in tomato leaves was identified and analysed. The lectin consists of two homologous chitin-binding modules interconnected by a short proline-rich domain containing a single Ser[Pro]( n ) repetitive motif. Each module comprises two in-tandem-arrayed hevein domains separated by a tetrapeptide linker. Besides the chitin-binding modules and proline-rich d...

Introduction Background: Considering the poorly understood mechanism of lysine demethylase 1A (KDM1A) in osteosarcoma (OS), we here commence our investigation to fill blank. Material and methods Methods: Following transfection as appropriate, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) flow cytometry assays were used determine viability apoptosis OS cells MG-63, which ge...