background: rotaviruses cause diarrhea in infants and young children worldwide. rotavirus outer capsid protein, vp7 is major neutralizing antigen that is important component of subunit vaccine to prevent rotavirus infection. many efforts have been done to produce recombinant vp7 that maintain native characteristics. we used baculovirus expression system to produce rotavirus vp7 protein and to s...

background: ferroportin (fpn), a regulator of iron homeostasis is a conserved membrane protein that exports iron across the enterocytes, macrophages and hepatocytes into the blood circulation. fpn has also critical influence on survival of microorganisms whose growth is dependent upon iron, thus preparation of fpn is needed to study the role of iron in immunity and pathogenesis of micoorganisms...

trauma and stressor related disorders include disorders in which exposure to a traumatic or stressful event is listed explicitly as a diagnostic criterion. these include reactive attachment disorder, posttraumatic stress disorder (ptsd), acute stress disorder, and adjustment disorders. psychological distress following exposure to a traumatic or stressful event is quite variable. in some cases, ...

conclusions the obtained results indicate that rsm is an effective method for the optimization of culture conditions for hes gene expression in e. coli. results a maximum rhes mrna level of 376.16% was obtained under the following conditions: 0.6 mm iptg, 7 hours post-induction time, and 0.9 cell density before induction. the level of rhes mrna was significantly correlated with post-induction t...

objective: to evaluate the effect of cytoplasm transfer from mature oocytes to germinal vesicle(gv)s on promoting the maturation of cytoplasm of gv at the mrna level. materials and methods: sixty six in vitro fertilization (ivf) operations between june 2012 and november 2013 were included in this study. totally 120 gvs were obtained. normal gvs were categorized into 3 groups (n = 40) randomly: ...

background and aims: the e2f family of transcription factors is encoded by at least eight genes, e2f1-8. these proteins are important targets of the retinoblastoma protein (rb) contributed in regulation of transcription, cell cycle and apoptosis. the aim of this study was to investigate the expression levels of e2f protein family including e2f1, e2f2, e2f7, e2f8 and rb1 in the lineage negative ...

background: influenza virus is the major cause of lower respiratory tract illnesses on the worldwide. vaccination can be an effective tool to prevent its outbreak. highly conserved viral nucleoprotein is an effective vaccine candidate to provide heterosubtypic immunity, offering resistance against various influenza virus strains. m aterials and methods: in present research np gene was inserted ...

background: with the increasing concern for health and nutrition, dietary fat has attracted considerable attention. the composition of fatty acids in the diet is important because they are associated with major diseases including cancers, diabetes, and cardiovascular disease. the fatty acid synthase (fasn) and stearoyl-coa desaturase (delta-9-desaturase) (scd) genes affect fatty acid compositio...

background: as the third most frequent cause of cancer death, breast cancer is a common disease worldwide. most of the patients are being diagnosed in the stage that conventional treatments are not effective, and invasion and metastases lead to death. therefore, identification of novel molecular markers to improve early diagnosis, prognosis and treatment of the breast cancer is a necessity. zin...

introduction: language impairment is a primary characteristic of children with autism spectrum disorder (asd). however, findings of language deficits in these children have been inconclusive, and many researchers believe that in such children the pattern of language profiles is different from normal children. to determine this pattern, comprehension and expression of syntax were examined in 10 ...