Traditional Chinese medicine (TCM) has an ancient history and unique system, including theory, methodology, prescription, formulation and medicines. There are differences between TCM and chemical drugs. For TCM, the multiple components in vivo are possibly to be detected; the number of components is relatively restricted; they could represent the therapeutic effect of the parent recipe; the con...

Analysis of pharmaceuticals and biomolecules using HPLC coupled to ICPMS and ESI-MS Andrew James Cartwright The work described within this thesis explores the use of HPLC coupled with ICPMS and ESI-MS in order to develop novel methods which overcome specific analytical challenges in the pharmaceutical industry. A membrane desolvation interface has been evaluated for coupling high performance li...

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BACKGROUND Opuntia dillenii is an invasive plant well established in the harsh South-Eastern arid zone of Sri Lanka. Evidence suggests it is likely that the endophytic fungal populations of O. dillenii assist the host in overcoming biotic and abiotic stress by producing biologically active metabolites. With this in mind there is potential to discover novel natural products with useful biologica...

When genome sections of wild Solanum species are bred into the cultivated potato (S. tuberosum L.) to obtain improved potato cultivars, the new cultivars must be evaluated for their beneficial and undesirable traits. Glycoalkaloids present in Solanum species are known for their toxic as well as for beneficial effects on mammals. On the other hand, glycoalkaloids in potato leaves provide natural...

Title of Dissertation: SYNTHETIC ION CHANNELS FROM LIPOPHILIC GUANOSINE DERIVATIVES Ling Ma, Doctor of Philosophy, 2009 Dissertation directed by: Professor Jeffery T. Davis Department of Chemistry and Biochemistry Synthetic ion channels and pores not only represent models of natural transmembrane ion channels, but also demonstrate their potential applications in the areas of drug delivery, bios...

Since Anfinsen’s famous experiments in the 1960s, it has been known that the complex three‐ dimensional structure of protein molecules is encoded in their amino acid sequences, and the chains autonomously fold under proper conditions. Cracking this code, which is sometimes called ‘‘the second part of the genetic code,’’ has been one of the greatest challenges of molecular biology. Although a fu...

A simple and rapid ultra-performance liquid chromatography-electrospray ionization mass spectrometry method for the simultaneous determination of thirteen free fatty acids (FFAs) in Pheretima has been developed and validated. Measurements for each FFA were linear over a wide range (0.05–3.95 μg mL) with good correlation coefficients (>0.99). The limit of detection and limit of quantification fo...

The potential of mass spectrometric peptide identification in complex mixtures by means of peptide mass fingerprinting (PMF) and peptide fragment fingerprinting (PFF) was evaluated and compared utilizing synthetic mixtures of commercially available proteins and electrospray-ion trapor electrospray time-of-flight mass spectrometers. While identification of peptides by PFF is fully supported by a...

We report the synthesis, structural, and electronic characterization of the theoretically predicted, but experimentally elusive δ-isomer of the Keggin polyanion. A family of δ-Keggin polyoxoanions of the general formula, (TEA)HpNaq [H2M12(XO4)O33(TEA)]rH2O where p, q, r = [2,3,8] for 1 and [4,1,4] for 2 were isolated by the reaction of tungstate(VI) and vanadium(V) with triethanolammonium ions ...