Abstract We describe a method for cleaning leaf epicuticular wax and contaminants from herbarium specimens in preparation scanning electron microscopy. Specimens are rinsed chloroform, heated at 50°C 8 hrs, ultrasonicated 70% ethanol, subjected to critical-point drying. This helps preserve reveal the surface details of that heavily covered with contaminants, making many morphologically function...

Wax esters are major constituents of the surface lipids in many terrestrial arthropods, but their study is complicated by their diversity. We developed a procedure for quantifying isomers in mixtures of straight-chain saturated and unsaturated wax esters having the same molecular weights, using single-ion monitoring of the total ion current data from gas chromatography-mass spectrometry. We exa...

Abstract Background Epicuticular wax (EW) is the first line of defense in plants for protection against biotic and abiotic factors environment. In wheat, EW associated with resilience to heat drought stress, however, current limitations on phenotyping restrict integration this secondary trait into wheat breeding pipelines. study we evaluated use light reflectance as a proxy load developed an ef...

The present survey of leaf-blade trichomes Mendoncia used SEM and light microscopy to investigate the diversity trichome types in Neotropical Paleotropical species genus. eglandular are filiform, uniseriate, with asymmetric epidermal cells arranged radially around trichome, these may be short or elongated, oriented two groups. These cells, when covered epicuticular wax, forming smooth thick pla...