We present a rate-determination method for analyzing glucose. A glucose enzyme electrode serves as the sensor and is made by placing a gel-immobilized layer of glucose oxidase over the tip of a Clark-type O2 electrode. The electrode membrane is made of Teflon and is derivatized by etching with a suspension of colloidal sodium metal in organic solvent. The enzyme is coupled to the membrane surfa...

Forty years have passed since Clark and Lyons proposed the concept of glucose enzyme electrodes. Excellent economic prospects and fascinating potential for basic research have led to many sensor designs and detection principles for the biosensing of glucose. Indeed, the entire field of biosensors can trace its origin to this glucose enzyme electrode. This review examines the history of electroc...

Lactate detection by an in situ sensor is of great need in clinical medicine, food processing, and athletic performance monitoring. In this paper, a flexible, easy to fabricate, and low-cost biosensor base on lactate oxidase is presented. The fabrication processes, including metal deposition, sol-gel IrOx deposition, and drop-dry enzyme loading method, are described in detail. The loaded enzyme...

The endoplasmic reticulum (ER) possesses a protein quality control system that supports the efficient folding of newly synthesized glycoproteins. In this system, a series of N-linked glycan intermediates displayed on proteins serve as quality tags. The ER folding-sensor enzyme UDP-glucose:glycoprotein glucosyltransferase (UGGT) operates as the gatekeeper for ER quality control by specifically t...

Heart failure is one of the leading causes death and disability worldwide. Left ventricle remodeling, fibrosis, ischemia/reperfusion injury all contribute to deterioration cardiac function predispose onset heart failure. Adenosine monophosphate-activated protein kinase (AMPK) universally recognized energy sensor which responds low ATP levels restores cellular metabolism. AMPK activation control...

Wireless sensor networks are often located in areas where access to them is difficult or dangerous. Today, in wireless sensor networks, cluster-based routing protocols by dividing sensor nodes into distinct clusters and selecting local head-clusters to combine and send information of each cluster to the base station and balanced energy consumption by network nodes, get the best performance ...

Oxidative stress can cause DNA breaks which induce activation of the DNA nick sensor enzyme poly(ADP-ribose) polymerase-1 (PARP-1), part of the 17 member PARP enzyme family. PARP-1 modifies target proteins by attaching to them several NAD-derived ADP-ribose units forming poly(ADP-ribose) (PAR) polymers. PARylation controls many cellular processes while intense PARylation may also lead to cell d...