Protein crystals have many important applications in many fields, including pharmaceutics. Being more stable than other formulations, and having a high degree of purity and bioavailability, they are especially promising in the area of drug delivery. In this contribution, the development of a continuously operated tubular crystallizer for the production of protein crystals has been described. Us...

The reaction of glucose with ferricyanide catalyzed by glucose oxidase from Aspergillus niger gives rise to a wide range of bistability as the flow rate is varied in a continuous flow stirred tank reactor. Oscillations in pH can be obtained by introducing a negative feedback on the autocatalytic production of H+ that drives the bistability. In our experiments, this feedback consists of an inflo...

enzymatic acidolysis of canola oil through caprylic and capric acids was investigated for making structured lipids. lipozyme tl im, an sn-1,3 specific thermomyce slanuginosa lipase, was utilized as the biocatalyst in the batch reactor. reaction conditions were designed according to taguchi’s method considering fatty acid to oil ratio, at three levels (1:1, 2:1, 3:1) , enzyme load (4, 8 and, 12%...

Triblock copolymeric nanoreactors are introduced as an alternative for liposomes as encapsulating carrier for prodrug activating enzymes. Inosine-adenosine-guanosine preferring nucleoside hydrolase of Trypanosoma vivax, a potential prodrug activating enzyme, was encapsulated in nanometer-sized vesicles constructed of poly(2-methyloxazoline)-block-poly(dimethylsiloxane)-block-(2-methyloxazoline)...

The rate of oxygen transfer from the gas phase to liquid is a critical process parameter for biocatalytic oxidations due poor water solubility molecular and low affinity many relevant enzymes, such as oxidases. In gas–liquid systems, mechanical mixing can be used increase interfacial area available mass thereby volumetric coefficient (kLa). As such, operation these reactions in continuous stirr...