نتایج جستجو برای: enzyme purification

تعداد نتایج: 289560  

Journal: :Applied and Environmental Microbiology 1998

Journal: :Agricultural and Biological Chemistry 1981

Ali Asghar Karkhane, Farid Heidari, Masoud Mashhadi Akbar Boojar, Meysam Rezaie, Saeed Aminzadeh,

Background: Typically, non-cellulytic glucanase, including fungi and yeast cell wall hydrolyzing enzymes, are released by some symbiotic fungi and plants during the mycoparasitic fungi attack on plants. These enzymes are known as the defense mechanisms of plants. This study intends to investigate the biochemical properties of β-1,6-glucanase (bg16M) from native thermophilic bacteria, Cohne...

Journal: :The Tohoku Journal of Experimental Medicine 1958

Journal: :Protein expression and purification 2013
Yonca Avci Duman Erdem Kaya

In this study chick pea β-galactosidase was first time purified and recovered in single step by three phase partitioning (TPP). Optimal purification parameters for TPP were 60% ammonium sulfate saturation (w/v) with 1:0.5 (v/v) ratio of crude extract:t-butanol at pH 6.8, which gave 10.1 purification fold with 133% recovery of β-galactosidase. SDS-PAGE analysis showed that protein has two subuni...

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