The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA s...

An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...

A simple and Reliable Bench top microscope is constructed to detect the fluorescence intensity for quantum dot. Which can act as fluorescent tag a sensitive studies like sandwich enzyme-linked immunosorbent assay (ELISA). The Cadmium Telluride (CdTe) capped with MPA (3-Mercaptopropionic acid) dot signal output (Excitation: 490 nm, Emission: 515 nm) used. It bifunctional molecule containing both...

The results of a field survey some potato growing areas in Nineveh Governorate showed the presence Potato virus s PVS, which was detected by Double Antibody Sandwich Enzyme-Linked ImmunoSorbent Assay DAS-ELISA test. This record is first governorate. Through absorbance readings using elisa reader, highest value recorded Sherikhan area samples, amounted to 1.190 nm, while lowest Rabia samples wit...

Objective: The aim of this study was to determine the seroprevalence Toxoplasma gondii (T. gondii) in schizophrenia patients and reveal importance toxoplasmosis as a risk factor these patients. Method: conducted on 190 diagnosed with schizophrenia. Also included control group were 100 healthy individuals without Anti-T. immunoglobulin G (IgG) IgM antibody levels determined via enzyme-linked imm...