Self-programmed enzyme phase separation is exploited to assemble dynamic multiphase coacervate droplets via spontaneous polyion self-sorting under non-equilibrium conditions.

Thiolated polymers are widely used in hydrogels for drug delivery, tissue engineering, and biofabrication. The oxidation of thiols is spontaneous, resulting the formation disulfide bridges cross-linking polymers. process is, however, difficult to control initiated directly when thiolated components exposed ambient conditions, which significantly complicates handling materials. Here, we show a f...

fumigant toxicity of rosemary essential oil on abamectin-resistant vs. susceptible populations of two spotted spider mites, tetranychus urticae koch was studied under laboratory conditions. results indicated that fumigant toxicity of rosemary essential oil on the susceptible population was 3.06 times higher than that of the resistant one. accordingly, there was a slight cross resistance observe...

The original proposal of Saier stating that P-enolpyruvate-dependent mannitol phosphorylation is catalyzed by the monomeric form of the bacterial phosphotransferase enzyme IImtl, which would be the form predominantly existing in the phospholipid bilayer, whereas mannitol/mannitol-P exchange would depend on the transient formation of functional dimers, is refuted [Saier, M.H. (1980) J. Supramol....

There have been recent renewed interests in the importance of suicide enzyme inhibition. The principal objective this review is to investigate all types inhibitions for natural enzymes, artificial biocatalysts as well therapeutic potential It discussed inhibition beneficial drug design and treatments non-beneficial achievements some industrial enzymes such HRP peroxidase enzyme. biomimetic expl...

The activity of Prunus dulcis (sweet almond) β-glucosidase at the expense of p-nitrophenyl-β-d-glucopyranoside at pH 6 was determined, both under steady-state and pre-steady-state conditions. Using crude enzyme preparations, competitive inhibition by 1-5 mM imidazole was observed under both kinetic conditions tested. However, when imidazole was added to reaction mixtures at 0.125-0.250 mM, we d...

Magnetic silica aerogel in hydrophobic and hydrophilic forms were used as support to immobilize Candida rugosa lipase by adsorption method. Response surface methodology (RSM) was employed to study the effects of the three most important immobilization parameters, namely enzyme/support ratio (0.3-0.5, w/w), immobilization time (60-120 min) and alcohol percentage (20-40, %v/v) on the specific act...