Horseshoe crabs (Limulus polyphemus and Tachypleus tridentatus) possess a proteolytic blood coagulation system within their amebocytes that, after release and endotoxin activation, generates a polymerized insoluble coagulin clot. Clotting enzyme from horseshoe crab amebocyte lysate is the protease that activates the clottable protein (coagulogen) which then forms the coagulin clot. Comparison o...

Graphical Abstract „The most important future applications of my research are the implementation more sustainable and efficient synthetic routes based on novel biocatalytic reactions that we have contributed to developing, discovery new enzyme-catalyzed … My favorite example chemistry in everyday life is beer fermentation! “ Find out about Marc Garcia-Borràs his Introducing Profile.

A fascinating group of enzymes has been shown to possess multiple active sites connected by intramolecular tunnels for the passage of reactive intermediates from the site of production to the site of utilization. In most of the examples studied to date, the binding of substrates at one active site enhances the formation of a reaction intermediate at an adjacent active site. The most common inte...

Environmentally friendly processes are nowadays a trending topic to get highly desired chemical compounds and, in this sense, the use of enzyme-catalyzed routes is becoming promising alternative traditional synthetic methods. In present paper, hybrid quantum mechanics/molecular mechanics (QM/MM) computational study on epoxidation alkenes catalyzed by Ser105Ala variant promiscuous Candida antarc...

The in vitro instability of the phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase [DAHPS(Phe)] from Escherichia coli has been found to be due to a metal-catalyzed oxidation mechanism. DAHPS(Phe) is one of three differentially feedback-regulated isoforms of the enzyme which catalyzes the first step of aromatic biosynthesis, the formation of DAHP from phosphoenolpyruvat...