Three simple immunological tests, the modified Elek (Biken) test, the modified staphylococcal coagglutination test, and the rapid GM1-horseradish peroxidase-enzyme-linked immunosorbent assay have been evaluated for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli. Of the 100 coded E. coli strains tested, 94 gave consistent results with all the three immunological tests; ...

The microbiota is a major source of protection against intestinal pathogens; however, the specific bacteria and underlying mechanisms involved are not well understood. As a model of this interaction, we sought to determine whether colonization of the murine host with symbiotic non-toxigenic Bacteroides fragilis could limit acquisition of pathogenic enterotoxigenic B. fragilis We observed strain...

A spontaneously occurring field isolate of enterotoxigenic Escherichia coli that was genotypically K99+ but phenotypically K99- was analyzed for the reason that it did not express K99. The defect, which was cis active, was located within an area 5' to the first gene required for K99 biogenesis and was the result of the deletion of a single base pair.

In this study, 210 intestinal Escherichia (E.) coli isolates from enteropathogenic diseased pigs were screened for their virulence factors (F4, F5, F6, F18, STa, STb, LT, STx2e and intimin) by PCR and serogrouped by slide-agglutination (O8:K87, O45, O138:K81, O139:K82, O141:K85, O147:K89, O157 and O149:K91). In total, 24.8% enterotoxigenic E. coli, 7.1% enteropathogenic E. coli and 5.2% oedema ...

Human and porcine enterotoxigenic strains of Escherichia coli were cultivated in tryptone-yeast extract medium or brain heart infusion broth and tested for production of heat-labile enterotoxin by the GM1 ganglioside enzyme-linked immunosorbent assay (GM1-ELISA) and the Y1 adrenal cell assay. When testing for enterotoxigenicity by the GM1-ELISA technique, homologous antisera for human and porci...

The production of staphylococcal enterotoxin A and staphylococcal enterotoxin B was investigated in S. aureus isolates obtained from milk of clinically healthy sheep. In comparison with valid standard (up to 1. 10 CFU. ml), the S. aureus counts were higher in 36 of the total number of 46 positive milk samples (ranging from 1.2. 10 to 8.2. 10 CFU. ml). Six (13.0%) of the 46 isolates were enterot...

Synthetic oligonucleotides, complementary to unique sequences in the heat stable enterotoxin gene of Escherichia coli specific for humans, were prepared with a 30-atom spacer arm and a 3' terminal sulfhydryl group which was coupled to bromoacetyl-derivatized alkaline phosphatase. The resulting direct enzyme-linked oligonucleotide probes, containing one enzyme molecule per oligonucleotide, succe...

Enterotoxigenic Escherichia coli (ETEC) is an important cause of bacterial diarrheal illness, affecting practically every population worldwide, and was estimated to cause 120,800 deaths in 2010. Here, we report the genome sequence of ETEC strain FMU073332, isolated from a 25-month-old girl from Tlaltizapán, Morelos, México.

INTRODUCTION Intestinal Escherichia coli pathogens are major causes of diarrhoeal disease in children under five years of age worldwide. The aim of this study is to evaluate the association of E. coli pathotypes with childhood diarrhoea in Colombia. METHODOLOGY A case-control study was conducted in 815 samples from children younger than five years of age in Cartagena, Colombia (466 cases and ...